Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

Scientists employed a multicellular 3D neurovascular unit organoid containing human brain microvascular endothelial cells, pericytes, astrocytes, microglia, oligodendrocytes and neurons to model the effects of hypoxia and neuroinflammation on blood-brain barrier function.
[Scientific Reports]
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Organoid Models for Mammary Gland Dynamics and Breast Cancer

The authors summarized key aspects of mammary gland biology and corresponding organoid models. The investigators also discussed emerging applications where organoids will be transformative for studying both the biology and diseases of the breast.
[Current Opinion in Cell Biology]
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Wnt/β‐Catenin Signaling Is Critical for Regenerative Potential of Distal Lung Epithelial Progenitor Cells in Homeostasis and Emphysema

Researchers identified an organoid‐forming adult distal lung epithelial progenitor cell population characterized by a low Wnt/β‐catenin activity, which was enriched in club and alveolar epithelial type II cells.
[Stem Cells]
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Organoid Polymer Functionality and Mode of Klebsiella pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells2

Polyethylene glycol-based immune organoids were developed to elucidate the effects of polymer end-point chemistry, integrin ligands, and mode of K. pneumoniae antigen presentation on germinal center-like B cell phenotype and epigenetics, to better define the lymph node microenvironment factors regulating ex vivo germinal center dynamics.
[Advanced Functional Materials]
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Hair-Bearing Human Skin Generated Entirely from Pluripotent Stem Cells

Scientists report an organoid culture system that generated complex skin from human PSCs. They used stepwise modulation of the transforming growth factor β and fibroblast growth factor signaling pathways to co-induce cranial epithelial cells and neural crest cells within a spherical cell aggregate.
[Nature]
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Bile Acids Signal via TGR5 to Activate Intestinal Stem Cells and Epithelial Regeneration

Researchers studied the role of bile acids and G protein-coupled bile acid receptor 1 (TGR5) in intestinal renewal, and regulation of intestinal stem cell function in mice and intestinal organoids.
[Gastroenterology]
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Cerebral Organoids: A Human Model for AAV Capsid Selection and Therapeutic Transgene Efficacy in the Brain

In cerebral organoids, transduction with recombinant adeno associated virus (rAAV)5 led to higher levels of vector DNA, transgenic mRNA and protein expression as compared to rAAV9. The superior transduction of rAAV5 was replicated in iPSC-derived neuronal cells.
[Molecular therapy-Nucleic Acids]
Depla, J. A., Sogorb-Gonzalez, M., Mulder, L., Heine, V. M., Konstantinova, P., Deventer, S. J. van, Wolthers, K. C., Pajkrt, D., Sridhar, A., & Evers, M. M. (2020). Cerebral organoids: a human model for AAV capsid selection and therapeutic transgene efficacy in the brain. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2020.05.028 Cite
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NitroSynapsin Ameliorates Hypersynchronous Neural Network Activity in Alzheimer hiPSC Models

Scientists developed a platform of physiological assays using patch-clamp electrophysiology, calcium imaging, and multielectrode array experiments on human (h)iPSC-derived 2D cortical neuronal cultures and 3D cerebral organoids.
[Molecular Psychiatry]
NitroSynapsin ameliorates hypersynchronous neural network activity in Alzheimer hiPSC models | Molecular Psychiatry. (n.d.). Retrieved June 4, 2020, from https://www.nature.com/articles/s41380-020-0776-7 Cite
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Brain Organoids: An Ensemble of Bioassays to Investigate Human Neurodevelopment and Disease

The authors summarize how the diverse brain organoid methods can be utilized to enhance our understanding of brain disorders.
[Cell Death and Differentiation]
Brain organoids: an ensemble of bioassays to investigate human neurodevelopment and disease | Cell Death & Differentiation. (n.d.). Retrieved June 4, 2020, from https://www.nature.com/articles/s41418-020-0566-4 Cite
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Cerebral Organoids: A Human Model for AAV Capsid Selection and Therapeutic Transgene Efficacy in the Brain

In cerebral organoids, transduction with recombinant adeno associated virus (rAAV)5 led to higher levels of vector DNA, transgenic mRNA and protein expression as compared to rAAV9. The superior transduction of rAAV5 was replicated in iPSC-derived neuronal cells.
[Molecular therapy-Nucleic Acids]
Depla, J. A., Sogorb-Gonzalez, M., Mulder, L., Heine, V. M., Konstantinova, P., Deventer, S. J. van, Wolthers, K. C., Pajkrt, D., Sridhar, A., & Evers, M. M. (2020). Cerebral organoids: a human model for AAV capsid selection and therapeutic transgene efficacy in the brain. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2020.05.028 Cite
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NitroSynapsin Ameliorates Hypersynchronous Neural Network Activity in Alzheimer hiPSC Models

Scientists developed a platform of physiological assays using patch-clamp electrophysiology, calcium imaging, and multielectrode array experiments on human (h)iPSC-derived 2D cortical neuronal cultures and 3D cerebral organoids.
[Molecular Psychiatry]
NitroSynapsin ameliorates hypersynchronous neural network activity in Alzheimer hiPSC models | Molecular Psychiatry. (n.d.). Retrieved June 4, 2020, from https://www.nature.com/articles/s41380-020-0776-7 Cite
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