pancreatic cells

[Communications Biology] Investigators reported the development of a high-throughput RNAi screening approach to identify upstream pathways that regulated adult human β cell quiescence and demonstrated in a screen of the GPCRome that silencing G-protein coupled receptor 3 (GPR3) led to human pancreatic β cell proliferation.

[Nature Communications] Scientists found that the γ-cell population was heterogeneous, with subsets of cells producing another hormone in addition to pancreatic polypeptide (Ppy).

[Islets] Scientists re-examined the performance of Hnf1b-CreERT2 with a Rosa26-RFP reporter transgene, which showed inducible recombination of up to 96% adult duct cells.

[Life Science Alliance] Reseachers showed that non-autologous exosomes could encapsulate CRISPR/Cas9 plasmid DNA via commonly available transfection reagents and could be delivered to recipient cancer cells to induce targeted gene deletion.

[American Journal of Physiology-Endocrinology and Metabolism] Cells with the most functional connections tended to activate sooner, have longer active times, and deactivated later. Scientists provided a common ground for recent differing views on beta cell heterogeneity.

[Journal of Proteome Research] The impact of DNAJA1 expression on pancreatic ductal adenocarcinoma progression remains unclear. The metabolic impacts of increased DNAJA1 expression were evaluated using a combination of untargeted metabolomics, stable isotope-resolved metabolomics, confocal microscopy, flow cytometry, and cell-based assays.