pulmonary cells

[iScience] Postnatal YAP activation increased epithelial cell proliferation, increased alveolar type-1 cell numbers, and caused indeterminate differentiation of subsets of alveolar cells expressing atypical genes normally restricted to airway epithelial cells.

[American Journal of Physiology-Lung Cellular and Molecular Physiology] Airway epithelial cells were obtained from tracheal aspirates (TA) from preterm newborns, and from bronchoalveolar lavage (BAL) or bronchial tissue (BT) from adults. TA and BAL cells were 3D-expanded, whereas cells from BT were expanded in 3D and 2D.

[Chemistry & Biodiversity] A549 and H1299 non-small lung cancer cell types were used for cytotoxicity using SRB and ATP assays. Fluorescent staining and M30 antigen assay were carried out for cell death evaluation.

[Nature Communications] Scientists investigated the oncogene-specific dependencies conferred by the lung cancer oncogene, RIT1. Genome-wide CRISPR screening in KRAS, EGFR, and RIT1-mutant isogenic lung cancer cells identifies shared and unique vulnerabilities of each oncogene..

[Cell Death & Disease] Scientists demonstrated that fibroblast growth factor-binding protein 1 was dramatically upregulated in airway epithelial cell lines treated by poly-L-arginine, a mimic of major basic protein.

[Scientific Reports] Primary human airway epithelial cells, cultured at air–liquid interface, were obtained by clinical airway brushings or from explanted lungs. Human airway Hb mRNA data were from publically available databases; or from RT-PCR.