reporter assay

[Breast Cancer Research] Researchers explored whether circCCDC85A could function as a miR-550a-5p sponge and influence breast cancer progression and found novel evidence that circCCDC85A inhibited the progression of breast cancer by functioning as a molecular sponge of miR-550a-5p to enhance MOB1A expression.

[Thoracic Cancer] Expression levels of circ_0007385, microRNA-493-3p (miR-493-3p) and Ras-related protein Rab-22A (RAB22A) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) in NSCLC tissues and cells.

[International Journal of Oncology] The expression and effect of miR‑1291 in colorectal cancer cells (CRC) was investigated. It was identified that miR‑1291 significantly suppressed the proliferation, invasion, cell mobility and colony formation of CRC cells.

[Journal of Gene Medicine] Expression of circular RNA derived from dipeptidyl peptidase 4 (circDPP4) was upregulated in prostate cancer (PC) tumors from 60 patients and PC cell lines, and higher circDPP4 might predict poor overall survival. Decreasing circDPP4 suppresses cell proliferation, colony formation, migration/invasion, and 50% inhibitory concentration of docetaxel in PC cells.

[Immunobiology] The authors explored the function and mechanism of LINC00184 in docetaxel resistance of prostate cancer and found that LINC00184 promoted docetaxel resistance and immune escape in prostate cancer cells by adsorption of miR-105-5p, resulting in upregulation of the expression of PD-L1.

[Environmental Toxicology] HK-2 cell viability and apoptosis were detected by Cell Counting Kit-8 assay and flow cytometry analysis, respectively. β-amyrin was found to ameliorate kidney injury in diabetic nephropathy mice and suppressed inflammatory response as well as apoptosis of HG-stimulated HK-2 cells.