single-cell RNA sequencing

[EMBO Reports] Scientists used cerebral organoids derived from patients and genome edited-iPSCs to address pathophysiological changes associated with a complex malformation of human cortical development caused by mutations in the echinoderm microtubule-associated protein-like 1 (EML1) gene.

[Molecules and Cells] Investigators gained insight into the cell type-specific transcriptomic changes by performing unbiased single-cell RNA sequencing of placental tissue samples, including those of patients diagnosed with preeclampsia and matched healthy controls.

[Nature Communications] Researchers used single-cell RNA sequencing of Drosophila medulla neuroblasts of all ages and identified a list of previously unknown temporal transcription factors, and experimentally characterized their roles in temporal patterning and neuronal specification.

[Journal of Hepatology] Scientists identified a proinflammatory hepatocyte subpopulation that developed primarily from periportal hepatocytes and to a lesser extent from pericentral hepatocytes and played key immunoregulatory roles in endotoxemia.

[Communications Biology] The myoepithelial lineage of human breast is comprised of bipotent and multipotent progenitors in ducts and terminal duct lobular units. The authors assessed whether this heterogeneity impacted on oncogenic PIK3CA transformation.

[iScience] Scientists developed a method to generate photoreceptor cells (PRCs) from human iPSCs by introducing the transcription factors CRX and NEUROD1, which enabled them to generate induced photoreceptor-like cells (iPRCs) expressing PRC markers.