Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids

Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, researchers observed that outgrowth potential varied non-linearly with initial organoid size.
[Journal of Mammary Gland Biology and Neoplasia]
Wrenn, E. D., Moore, B. M., Greenwood, E., McBirney, M., & Cheung, K. J. (2020). Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids. Journal of Mammary Gland Biology and Neoplasia. https://doi.org/10.1007/s10911-020-09464-1 Cite
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Physicochemical Characterization and Self-Assembly of Human Amniotic Membrane and Umbilical Cord Collagen: A Comparative Study

Investigators extracted collagen from human amniotic membrane and umbilical cord, which are treated as medical waste and compared its physico-chemical properties.
[International Journal of Biological Macromolecules]
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Standardized Xeno- and Serum-Free Culture Platform Enables Large-Scale Expansion of High-Quality Mesenchymal Stem/Stromal Cells from Perinatal and Adult Tissue Sources

The authors established a standardized culture platform using xeno- and serum-free commercial media for expansion of MSCs derived from umbilical cord, bone marrow and adipose-derived and examined their functional characteristics.
[Cytotherapy]
Hoang, V. T., Trinh, Q.-M., Phuong, D. T. M., Bui, H. T. H., Hang, L. M., Ngan, N. T. H., Anh, N. T. T., Nhi, P. Y., Nhung, T. T. H., Lien, H. T., Nguyen, T. D., Thanh, L. N., & Hoang, D. M. (2020). Standardized xeno- and serum-free culture platform enables large-scale expansion of high-quality mesenchymal stem/stromal cells from perinatal and adult tissue sources. Cytotherapy, 0(0). https://doi.org/10.1016/j.jcyt.2020.09.004 Cite
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Enhancing the Therapeutic Potential of CCL2-Overexpressing Mesenchymal Stem Cells in Acute Stroke

After C–C motif chemokine ligand 2 (CCL2)-overexpressing human umbilical cord-derived MSCs were intravenously transplanted with mannitol in rats with middle cerebral arterial occlusion, scientists compared the differences between four different treatment groups: mannitol + CCL2-overexpressing hUC-MSCs, mannitol + naïve hUC-MSCs, mannitol only, and control.
[International Journal of Molecular Sciences]
Lee, S., Kim, O. J., Lee, K. O., Jung, H., Oh, S.-H., & Kim, N. K. (2020). Enhancing the Therapeutic Potential of CCL2-Overexpressing Mesenchymal Stem Cells in Acute Stroke. International Journal of Molecular Sciences, 21(20), 7795. https://doi.org/10.3390/ijms21207795 Cite
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Enumerating Regulatory T Cells in Cryopreserved Umbilical Cord Blood Samples Using FOXP3 Methylation Specific Quantitative PC

To establish the most robust and efficient way to measure the Treg content of previously cryopreserved cord blood units, researchers compared the enumeration of Treg and CD3+ cells using flow cytometry and an epigenetic, DNA-based methodology.
[PLoS One]
Duggleby, R. C., Tsang, H. P., Strange, K., McWhinnie, A., Lamikanra, A. A., Roberts, D. J., Hernandez, D., Madrigal, J. A., & Danby, R. D. (2020). Enumerating regulatory T cells in cryopreserved umbilical cord blood samples using FOXP3 methylation specific quantitative PCR. PLOS ONE, 15(10), e0240190. https://doi.org/10.1371/journal.pone.0240190 Cite
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Y-27632 Promotes the Healing of Corneal Endothelial Wounds Using Endothelial Progenitor Cells Derived from Umbilical Cord Blood

Scientists investigated the proliferation of umbilical cord blood–derived endothelial progenitor cells and the differentiation efficiency toward corneal endothelial cell-like cells induced by rho-associated protein kinase inhibitor Y-27632 and to determine the most effective strategy for repairing corneal endothelium injuries in rabbits.
[Cornea]
Zhang, W., Yu, F., Chen, J., Fu, Y., Shao, C., & Fan, X. (2020). Y-27632 Promotes the Healing of Corneal Endothelial Wounds Using Endothelial Progenitor Cells Derived From Umbilical Cord Blood. Cornea, Publish Ahead of Print. https://doi.org/10.1097/ICO.0000000000002560 Cite
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Indole Hydrazide Compound ZJQ-24 Inhibits Angiogenesis and Induces Apoptosis Cell Death through Abrogation of AKT/mTOR Pathway in Hepatocellular Carcinoma

MTT assays, flow cytometric analysis, Western blotting and immunohistochemistry identified that ZJQ-24 effectively suppressed hepatocellular carcinoma cell proliferation via G2/M phase arrest and caspase-dependent apoptosis but had no cytotoxic on normal cells.
[Cell Death & Disease]
Liu, J., Liu, Y., Zhang, J., Liu, D., Bao, Y., Chen, T., Tang, T., Lin, J., Luo, Y., Jin, Y., & Zhang, J. (2020). Indole hydrazide compound ZJQ-24 inhibits angiogenesis and induces apoptosis cell death through abrogation of AKT/mTOR pathway in hepatocellular carcinoma. Cell Death & Disease, 11(10), 1–13. https://doi.org/10.1038/s41419-020-03108-2 Cite
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G9a Plays Distinct Roles in Maintaining DNA Methylation, Retrotransposon Silencing, and Chromatin Looping

Scientists observed widespread DNA methylation loss in G9a depleted and catalytic mutant ESCs. Furthermore, they defined how G9a regulates chromatin accessibility, epigenetic modifications, and transcriptional silencing in both catalytic-dependent and -independent manners.
[Cell Reports]
Jiang, Q., Ang, J. Y. J., Lee, A. Y., Cao, Q., Li, K. Y., Yip, K. Y., & Leung, D. C. Y. (2020). G9a Plays Distinct Roles in Maintaining DNA Methylation, Retrotransposon Silencing, and Chromatin Looping. Cell Reports, 33(4). https://doi.org/10.1016/j.celrep.2020.108315 Cite
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CRISPR/Cas9 Mediated Generation and Analysis of N-Terminus Polymorphic Models of beta2-Adrenoreceptor in Isogenic hPSC Derived Cardiomyocytes

Investigators used CRISPR/Cas9 gene editing technology in human (h)PSCs to create a unique suite of four isogenic homozygous variants at amino acid positions 16(G/R) and 27(G/Q), which reside in the N-terminus of the β2AR.
[Molecular Therapy-Methods & Clinical Development]
Kondrashov, A., Yusof, N. A. N. M., Hasan, A., Goulding, J., Kodagoda, T., Hoang, D. M., Vo, N. T. N., Melarangi, T., Dolatshad, N., Gorelik, J., Hill, S., Harding, S. E., & Denning, C. (2020). CRISPR/Cas9 mediated generation and analysis of N-terminus polymorphic models of beta2-adrenoreceptor in isogenic hPSC derived cardiomyocytes. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2020.10.019 Cite
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A Multifunctional LNA Oligo-Based Strategy Blocks Androgen Receptor Expression and Transactivation Activity in Prostate Cancer Cells

The Androgen Receptor (AR) plays a critical role in the development of prostate cancer (PCa) through the activation of androgen-induced cellular proliferation genes. Using tailor-made splice switching Locked Nucleic Acid (LNA) oligos, scientists successfully redirected splicing of the AR pre-mRNA and destabilized the transcripts via the introduction of premature stop codons.
[Molecular Therapy-Nucleic Acids]
Castanotto, D., Zhang, X., Rüger, J., Alluin, J., Sharma, R., Pirrotte, P., Joenson, L., Ioannou, S., Nelson, M. S., Vikeså, J., Hansen, B. R., Koch, T., Jensen, M. A., Rossi, J. J., & Stein, C. A. (2020). A Multifunctional LNA Oligo-Based Strategy Blocks Androgen Receptor Expression And Transactivation Activity In Prostate Cancer Cells. Molecular Therapy - Nucleic Acids, 0(0). https://doi.org/10.1016/j.omtn.2020.10.032 Cite
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A Broad Response to Intracellular Long-Chain Polyphosphate in Human Cells

Polyphosphates (polyPs) are long chains of inorganic phosphates linked by phosphoanhydride bonds. They are found in all kingdoms of life, playing roles in cell growth, infection, and blood coagulation. Scientists demonstrated that internally produced polyP can activate diverse signaling pathways in human cells.
[Cell Reports]
Bondy-Chorney, E., Abramchuk, I., Nasser, R., Holinier, C., Denoncourt, A., Baijal, K., McCarthy, L., Khacho, M., Lavallée-Adam, M., & Downey, M. (2020). A Broad Response to Intracellular Long-Chain Polyphosphate in Human Cells. Cell Reports, 33(4). https://doi.org/10.1016/j.celrep.2020.108318 Cite
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