Tocilizumab Potentiates Cisplatin Cytotoxicity and Targets Cancer Stem Cells in Triple‐Negative Breast Cancer

Tocilizumab suppressed the stemness‐related characteristics of triple‐negative breast cancer cells, through the inhibition of the Wnt/β‐catenin breast cancer stem cell‐related pathway.
[Molecular Carcinogenesis]
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Single-Cell RNA-Seq Mapping of Human Thymopoiesis Reveals Lineage Specification Trajectories and a Commitment Spectrum in T Cell Development

Scientists used single-cell RNA sequencing to interrogate the rare CD34+ progenitor and the more differentiated CD34 fractions in the human postnatal thymus.
[Immunity]
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Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4°C

Researchers demonstrated that collecting and processing of human cord blood at 4°C within minutes of the baby’s birth results in significantly enhanced numbers of rigorously defined phenotypic hematopoietic stem cell and self‐renewing NSG immune‐deficient mouse engrafting and SCID‐repopulating cells.
[Stem Cells]
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Autism-Associated SHANK3 Mutations Impair Maturation of Neuromuscular Junctions and Striated Muscles

Investigators used a combination of patient-derived human iPSCs, Shank3Δ11(−/−) mice, and Phelan-McDermid syndrome muscle biopsies from patients of different ages to analyze the role of SHANK3 on motor unit development.
[Science Translational Medicine]
Autism-associated SHANK3 mutations impair maturation of neuromuscular junctions and striated muscles | Science Translational Medicine. (n.d.). Retrieved June 17, 2020, from https://stm.sciencemag.org/content/12/547/eaaz3267 Cite
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High-Throughput Intracellular Biopsy of microRNAs for Dissecting the Temporal Dynamics of Cellular Heterogeneity

Using intracellular biopsy technique (inCell-Biopsy), scientists analyzed the temporal miRNA transcriptome over the differentiation of ESCs. The combinatorial miRNA expression patterns derived by inCell-Biopsy identified emerging cell subpopulations differentiated from ESCs and revealed the dynamic evolution of cellular heterogeneity.
[Science Advances]
Wang, Z., Qi, L., Yang, Y., Lu, M., Xie, K., Zhao, X., Cheung, E. H. C., Wang, Y., Jiang, X., Zhang, W., Huang, L., Wang, X., & Shi, P. (2020). High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity. Science Advances, 6(24), eaba4971. https://doi.org/10.1126/sciadv.aba4971 Cite
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A lncRNA-miRNA-mRNA Network for Human Primed, Naive and Extended Pluripotent Stem Cells

Researchers successfully converted three in-house-derived primed human PSC lines (H10, H24, and iPS) to a naive state and an expanded pluripotent stem cell (EPS) state. Primed, naive and EPS cells displayed state-specific morphologies and expressed pluripotent markers.
[PLoS One]
Ma, Z., Li, Y., Zhang, Y., Chen, J., Tan, T., & Fan, Y. (2020). A lncRNA-miRNA-mRNA network for human primed, naive and extended pluripotent stem cells. PLOS ONE, 15(6), e0234628. https://doi.org/10.1371/journal.pone.0234628 Cite
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Simulating the Human Colorectal Cancer Microenvironment in 3D Tumor-Stroma Co-Cultures In Vitro and In Vivo

Researchers successfully converted three in-house-derived primed human PSC lines to a naive state and an expanded pluripotent stem cell (EPS) state. Primed, naive and EPS cells displayed state-specific morphologies and expressed pluripotent markers.
[Scientific Reports]
Devarasetty, M., Dominijanni, A., Herberg, S., Shelkey, E., Skardal, A., & Soker, S. (2020). Simulating the human colorectal cancer microenvironment in 3D tumor-stroma co-cultures in vitro and in vivo. Scientific Reports, 10(1), 9832. https://doi.org/10.1038/s41598-020-66785-1 Cite
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Mitochondrial Dysfunction Generates Aggregates That Resist Lysosomal Degradation in Human Breast Cancer Cells

Researchers demonstrated protein aggregates and autophagy receptors, TAX1BP1 and NDP52, as potential endpoints for monitoring autophagy during drug development and clinical studies.
[Cell Death & Disease]
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Rapid Cancer Diagnosis and Early Prognosis of Metastatic Risk Based on Mechanical Invasiveness of Sampled Cells

Investigators evaluated the mechanical invasiveness of established breast and pancreatic cell lines and of tumor-cells from fresh, suspected pancreatic cancer tumors. The mechanical invasiveness matched the in vitro metastatic potential in cell lines as determined with Boyden chamber assays.
[Annals of Biomedical Engineering]
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SphK2 Confers 5-Fluorouracil Resistance to Colorectal Cancer via Upregulating H3K56ac-Mediated DPD Expression

Investigators showed that SphK2high conferred 5-fluorouracil resistance through upregulated tumoral dihydropyrimidine dehydrogenase.
[Oncogene]
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Intratumoral Immune Activation with TLR4 Agonist Synergizes with Effector T Cells to Eradicate Established Murine Tumors

Scientists combined intratumoral administration of the synthetic toll-like receptor 4 agonist glucopyranosyl lipid A with either active vaccination or adoptive transfer of tumor-specific CD8 T cells to mice bearing established melanomas.
[Npj Vaccines]
Intratumoral immune activation with TLR4 agonist synergizes with effector T cells to eradicate established murine tumors | npj Vaccines. (n.d.). Retrieved June 16, 2020, from https://www.nature.com/articles/s41541-020-0201-x Cite
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