Publication

[Nature Methods] Researchers described single-cell Deep Visual Proteomics, a technology that integrated high-content imaging, laser microdissection, and multiplexed mass spectrometry.

[Nature Communications] The authors elucidated an endocrine pathway that was regulated by skeletal muscle interferon regulatory factor 4, which manipulated liver pathology.

[Cancer Letters] Researchers identified inhibition of reactive oxygen species levels and reduced redox status in Lenvatinib-resistant HCC. Integrating RNA-seq with unbiased whole-genome CRISPR-Cas9 screen analysis indicated LINC01607 regulated the P62 to enhance drug resistance by affecting mitophagy and antioxidant pathways.

[Biochemical Pharmacology] Scientists investigated a potential role of CRY1/2 in circadian regulation of hepatic Cytochromes P450 (CYP) 2A5. Regulatory effects of CRY1/2 on CYP2A5 were determined using cryptochrome 1(Cry1-null and Cry2-null mice, and validated using AML-12, Hepa1-6, and HepG2 cells.

[Journal of Biological Chemistry] The authors mapped the phosphorylation rewiring events in palmitic acid (PA)-stimulated HepG2 cells and found PA decreased the phosphorylation level of the eukaryotic translation initiation factor 4E-binding protein 4EBP2 at S65/T70.

[Communications Biology] Scientists showed that genetic deletion of protection of telomeres 1a (POT1a) in MSCs led to intracellular accumulation of fatty acids and excessive reactive oxygen species and DNA damage, resulting in impaired osteogenic-differentiation.