Publication

[Nature] Researchers developed the CRISPR–human organoids–single-cell RNA sequencing system, which used verified pairs of guide RNAs, inducible CRISPR–Cas9-based genetic disruption, and single-cell transcriptomics for pooled loss-of-function screening in mosaic organoids.

[Cell Stem Cell] Investigators reported that blastoids cultured on thick three-dimensional (3D) extracellular matrices captured hallmarks of early post-implantation development, including epiblast lumenogenesis, rapid expansion and diversification of trophoblast lineages, and robust invasion of extravillous trophoblast cells by day 14.

[Nature Communications] Scientists used human blood vessel organoids (BVOs) as a model of the microvasculature. BVOs fully recapitulated key features of the human microvasculature, including the reliance of mature endothelial cells on glycolytic metabolism.

[Science of The Total Environment] Investigators focused on the toxic effects of nanoplastics on brain developmental processes by investigating their interactions with brain organoids.

[Cell Proliferation] Scientists showed that apical-out organoids had drastically reduced proliferative potential, as evidenced by immunohistochemical staining and the incorporation of the thymidine analog EdU.

[Frontiers in Cell and Developmental Biology] For characterization of organoids’ response to single or multiple-cell ablation, researchers analyzed overall organoid survival and found that airway organoids were capable of efficiently repairing damage induced by femtosecond laser-based ablation of a single to ten cells within 24 h.