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western blot analysis

Autoantibody against Angiotensin II Type I Receptor Induces Pancreatic β-Cell Apoptosis via Enhancing Autophagy

[Acta Biochimica et Biophysica Sinica] INS-1 cells were treated with autoantibody against the angiotensin II type I receptor, and cell viability, apoptosis, and autophagy-related proteins were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and western blot analysis, respectively.

Patient-Specific induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells as a Model to Study Autosomal Recessive Hypercholesterolemia

[Stem Cells and Development] Investigators aimed to validate patient-specific iPSC-derived hepatocyte-like cells as an appropriate tool to model autosomal recessive hypercholesterolemia disease.

M2a Macrophage Can Rescue Proliferation and Gene Expression of Benign Prostate Hyperplasia Epithelial and Stroma Cells from Insulin-Like Growth Factor 1 Knockdown

[Prostate] Scientists explored the effect of M2a macrophages on the development of benign prostatic hyperplasia via insulin-like growth factor 1.

Novel Role of LINC01013/miR-6795-5p/FMNL3 Axis in the Regulation of Hepatocellular Carcinoma Stem Cell Features

[Acta Biochimica et Biophysica Sinica] Hepatocellular carcinoma tissues and normal controls were collected, and the expression pattern of LINC01013 and miR-6795-5p was identified by quick real-time polymerase chain reaction.

Protection of β-Pancreatic Cells From Dysfunctionality of Insulin Using Vitexin by Apoptosis of INS-1 Cells

[Archives of Physiology and Biochemistry] The authors explored the possible beneficial effects of vitexin on high glucose-induced cytotoxicity in pancreatic β-cells. The INS-1 pancreatic β-cell line was used in this study.

CGRP Protects Bladder Smooth Muscle Cells Stimulated By High Glucose through Inhibiting p38 MAPK Pathway In Vitro

[Scientific Reports] Researchers indicated that calcitonin gene-related peptide (CGRP) protected bladder smooth muscle cells from oxidative stress induced by high glucose in vitro, and inhibit the α-SM-actin expression decrease through inhibiting the intracellular p38 MAPK signaling pathway.

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