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western blot analysis
Pancreatic Cell News
Autoantibody against Angiotensin II Type I Receptor Induces Pancreatic β-Cell Apoptosis via Enhancing Autophagy
[Acta Biochimica et Biophysica Sinica] INS-1 cells were treated with autoantibody against the angiotensin II type I receptor, and cell viability, apoptosis, and autophagy-related proteins were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and western blot analysis, respectively.
ESC & iPSC News
Patient-Specific induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells as a Model to Study Autosomal Recessive Hypercholesterolemia
[Stem Cells and Development] Investigators aimed to validate patient-specific iPSC-derived hepatocyte-like cells as an appropriate tool to model autosomal recessive hypercholesterolemia disease.
Prostate Cell News
M2a Macrophage Can Rescue Proliferation and Gene Expression of Benign Prostate Hyperplasia Epithelial and Stroma Cells from Insulin-Like Growth Factor 1 Knockdown
[Prostate] Scientists explored the effect of M2a macrophages on the development of benign prostatic hyperplasia via insulin-like growth factor 1.
Cancer Stem Cell News
Novel Role of LINC01013/miR-6795-5p/FMNL3 Axis in the Regulation of Hepatocellular Carcinoma Stem Cell Features
[Acta Biochimica et Biophysica Sinica] Hepatocellular carcinoma tissues and normal controls were collected, and the expression pattern of LINC01013 and miR-6795-5p was identified by quick real-time polymerase chain reaction.
Pancreatic Cell News
Protection of β-Pancreatic Cells From Dysfunctionality of Insulin Using Vitexin by Apoptosis of INS-1 Cells
[Archives of Physiology and Biochemistry] The authors explored the possible beneficial effects of vitexin on high glucose-induced cytotoxicity in pancreatic β-cells. The INS-1 pancreatic β-cell line was used in this study.
Muscle Cell News
CGRP Protects Bladder Smooth Muscle Cells Stimulated By High Glucose through Inhibiting p38 MAPK Pathway In Vitro
[Scientific Reports] Researchers indicated that calcitonin gene-related peptide (CGRP) protected bladder smooth muscle cells from oxidative stress induced by high glucose in vitro, and inhibit the α-SM-actin expression decrease through inhibiting the intracellular p38 MAPK signaling pathway.