High Yield Secretion of Human Erythropoietin from Tobacco Cells for Ex Vivo Differentiation of Hematopoietic Stem Cells towards Red Blood Cells

To overcome the bottlenecks of low protein productivity and secretion, erythropoietin was expressed in tobacco BY-2 cells with a designer peptide tag, termed (SP)20 that consists of 20 tandem repeats of a “Ser-Pro” motif.