STRAIGHT-IN Enables High-Throughput Targeting of Large DNA Payloads in Human Pluripotent Stem Cells

The authors merged the strengths of different classes of site-specific recombinases and combined these with CRISPR-Cas9-mediated homologous recombination to develop a strategy for stringent site-specific replacement of genomic fragments at least 50 kb in size in hiPSCs.