STRAIGHT-IN Enables High-Throughput Targeting of Large DNA Payloads in Human Pluripotent Stem Cells

The authors merged the strengths of different classes of site-specific recombinases and combined these with CRISPR-Cas9-mediated homologous recombination to develop a strategy for stringent site-specific replacement of genomic fragments at least 50 kb in size in hiPSCs.
[Cell Reports Methods]
Full ArticleGraphical Abstract
spot_img

Stay up-to-date with your field!

Subscribe for free weekly science newsletters.

Related News