High-Throughput Functional Assay in Cystic Fibrosis Patient-Derived Organoids Allows Drug Repurposing

Scientists miniaturised the previously described forskolin-induced swelling assay in intestinal organoids from a 96-well to a 384-well plate screening format, and used this format to test CFTR increasing potential of a 1400-compound FDA-approved drug library in organoids from donors with W1282X/W1282X CFTR nonsense mutations.