Single-Cell RNA Sequencing Reveals Maturation Trajectory in Human Pluripotent Stem Cell-Derived Cardiomyocytes in Engineered Tissues

Researchers studied the cellular phenotype of human pluripotent stem cell-derived cardiomyocytes by comparing their single-cell gene expression and functional profiles in three engineered cardiac tissue configurations: human ventricular (hv) cardiac anisotropic sheet, cardiac tissue strip, and cardiac organoid chamber, with spontaneously aggregated 3D cardiac spheroids as control.