Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation during Human iPSC Differentiation

Researchers modulated WNT, Activin/Nodal, and MAPK signaling pathways by stage-specific addition of small molecule regulators CHIR99021, SB431542, and LY294002, respectively, and measured the impact on hematoendothelial formation in culture.