Truncated Vitronectin with E-Cadherin Enables the Xeno-Free Derivation of Human Embryonic Stem Cells

Three hESC lines were successfully derived on the vitronectin with a truncated N-terminus in combination with E-cadherin in xeno-free conditions for the first time, and their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers were confirmed.