Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Perioperative Neurocognitive Disorder by Inhibiting Inflammatory Responses and Activating BDNF/TrkB/CREB Signaling Pathway in Aged Mice

Human umbilical cord-MSCs were isolated from an infant umbilical cord and identified using flow cytometry and differentiation assays. Scientists established PND model by undergoing aseptic laparotomy under isoflurane anesthesia maintaining spontaneous ventilation in 18-month-old male C57BL/6 mice.