Towards Label-Free Standardization in 3D Cell Culture: Automated, Selective and Gentle High-Throughput Handling of Spheroids and Organoids via Novel Pick-Flow-Drop Principle

A novel method for fully automated size classification and handling of cell aggregates like spheroids and organoids was presented. Using microfluidic flow generated by a piezoelectric droplet generator, aggregates were aspirated from a reservoir on one side of a thin capillary and deposited on the other side, encapsulated in free-flying nanoliter droplets to a target.