HAND Factors Regulate Cardiac Lineage Commitment and Differentiation From Human Pluripotent Stem Cells

With NKX2.5eGFP H9 hESCs, investigators established single and double knockout cell lines for HAND1 and HAND2, respectively, whose cardiomyocyte differentiation efficiency could be monitored by assessing NKX2.5-eGFP+ cells with flow cytometry.