HAND Factors Regulate Cardiac Lineage Commitment and Differentiation From Human Pluripotent Stem Cells

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With NKX2.5eGFP H9 hESCs, investigators established single and double knockout cell lines for HAND1 and HAND2, respectively, whose cardiomyocyte differentiation efficiency could be monitored by assessing NKX2.5-eGFP+ cells with flow cytometry.
[Current Stem Cell Research & Therapy]
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