Development of KoRV-Pseudotyped Lentiviral Vectors for Efficient Gene Transfer Into Freshly Isolated Immune Cells

Koala Retrovirus-based envelope demonstrated remarkable efficiency in transducing freshly isolated primary human NK cells directly from blood, as well as freshly obtained monocytes, which were differentiated to M1 macrophages as well as B cells from multiple donors, achieving up to 80% reporter gene expression within three days post-transduction.