The Combination of Single-Cell and RNA Sequencing Analysis Decodes the Melanoma Tumor Microenvironment and Identifies Novel T Cell-Associated Signature Genes

Investigators utilized single-cell RNA sequencing to analyze 34 melanoma samples from two public datasets. They extracted 706 marker genes associated with immune checkpoint (ICP) therapy from these T cells, 509 markers of T cells from 11 melanoma tissues, and eventually identified 33 candidate genes.