Modeling Clonal Hematopoiesis in Umbilical Cord Blood Cells by CRISPR/Cas9

Site-specific mutations were introduced in defined regions of DNMT3A, TET2, and ASXL1 in CD34⁺ progenitor cells that were subsequently analyzed in short-term as well as long-term in vitro culture assays to assess self-renewal and differentiation capacities.