Scientists present a step-by-step methodology to generate human minibrain nurseries and novel strategies to subsequently label projection neurons, perform immunohistochemistry and 3D imaging of the minibrains at large multiplexable scales.
Govindan, S., Batti, L., Osterop, S. F., Stoppini, L., & Roux, A. (2021). Mass Generation, Neuron Labeling, and 3D Imaging of Minibrains. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.582650Cite
Angiogenesis and β‐cell expansion in the pancreas were coupled by a distinct age‐dependent subset of endothelial cells (ECs). While this EC subpopulation supported pancreatic β cells, it declined during aging concomitant with increased expression of the gap junction protein Gja1.
Chen, J., Lippo, L., Labella, R., Tan, S. L., Marsden, B. D., Dustin, M. L., Ramasamy, S. K., & Kusumbe, A. P. (2020). Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system. The EMBO Journal, n/a(n/a), e105242. https://doi.org/10.15252/embj.2020105242Cite
Kolanowski, T. J., Rozwadowska, N., Zimna, A., Nowaczyk, M., Siatkowski, M., Łabędź, W., Wiland, E., Gapiński, J., Jurga, S., & Kurpisz, M. (2020). Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes. Scientific Reports, 10(1), 14336. https://doi.org/10.1038/s41598-020-70756-xCite