ATR

[Cell Death & Differentiation] Researchers showed that the replication stress response was efficient in primary CSCs from colorectal cancer, and described unique roles for PARP1 and MRE11/RAD51.

[Scientific Reports] In in vitro studies, collagen type VI secretion from human renal glomerular endothelial cells was assessed by measuring the decrease in the cytoplasmic COL6-positive cells and an increase in the amount of COL6 in the culture medium.

[Scientific Reports] 5-AzadC induced HEXIM1 expression in prostate cancer cell lines and triple negative breast cancers. 5-AzadC-induced DNA damage enhanced P-TEFb occupancy via a mechanism that involved activation of ATR and ATM and induction of NF-ĸB recruitment to the HEXIM1 promoter.

[Molecular Cancer Research] Researchers showed that BGB324, a selective small-molecule AXL inhibitor, caused DNA damage and induced replication stress, indicated by ATR/CHK1 phosphorylation, more significantly in TP53-deficient non-small cell lung cancer cell lines.

[Scientific Reports] Using CRISPR/Cas9 in the colorectal cancer cell line DLD-1, which harbors four POLD1 variants, scientists established heterozygous POLD1-knockout clones with exclusive expression of distinct variants to determine the functional relevance of these variants individually by assessing their impact on ATR pathway activation, DNA replication, and cellular sensitivity to inhibition of ATR or its effector kinase CHK1.

[Gastroenterology] Scientists interrogated the transcriptome, genome, proteome and functional characteristics of 61 novel pancreatic cancer patient-derived cell lines to define novel therapeutic strategies targeting DDR and replication stress. Validation was done in patient derived xenografts and human PC organoids.