Scientists explore how self-amplifying RNAs are emerging as important vaccine candidates for infectious diseases, the advantages of synthetic manufacturing approaches, and their potential for preventing and treating chronic infections.
Scientists demonstrated that envelope glycoproteins (Env)-induced oxidative stress was responsible for their death by apoptosis. Moreover, they report that peroxisomes, organelles involved in the control of oxidative stress, are targeted by Env-mediated autophagy.
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Researchers describe an optimized laboratory-scale workflow whereby lentiviral vector (LV)-containing supernatant was purified and concentrated by sequential chromatographic steps, obtaining biologically active LVs with infectious titer and specific activity in the order of 109 Transducing Unit (TU)/ml and 5×104 TU/ng of HIV Gag p24, respectively.
[Molecular Therapy-Methods & Clinical Development]
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Soldi, M., Sergi, L. S., Unali, G., Kerzel, T., Cuccovillo, I., Capasso, P., Annoni, A., Biffi, M., Rancoita, P. M. V., Cantore, A., Lombardo, A., Naldini, L., Squadrito, M. L., & Kajaste-Rudnitski, A. (2020). Laboratory-scale lentiviral vector production and purification for enhanced ex vivo and in vivo genetic engineering. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2020.10.009 Cite
Orion Biotechnology Canada Ltd. announced the results of their Phase I study of OB-002H which is an investigational candidate microbicide for HIV prevention.
[Orion Biotechnology Canada Ltd.]
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HIV-unexposed (HU) and HIV-exposed uninfected (HEU) infant cord blood monocytes were treated with Bacillus Calmette-Guerín (BCG) and transcriptionally profiled with the Nanostring nCounter platform. Differential expression and pathway enrichment analyses were performed, and transcripts were identified with enhanced or dampened BCG responses.
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The authors discuss recent advancements regarding the signals and transcription factors regulating germinal centre-derived memory B cells development and function.
[Nature Reviews Immunology]
Scientists reconstituted processes of reverse transcription and concerted integration in a cell-free system, using purified HIV-1 virions as the source of viral genomes and enzymes.
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Scientists discovered that exposure of rat primary neurons to Tat resulted in the up-regulation of an uncharacterized long non-coding RNA (lncRNA), LOC102549805.
[Cell Death & Disease]
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Scientists investigated the patterns of T cell escape variants in the replication-competent reservoir of 25 persons living with HIV-1 durably suppressed on ART.
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Warren, J. A., Zhou, S., Xu, Y., Moeser, M. J., MacMillan, D. R., Council, O., Kirchherr, J., Sung, J. M., Roan, N., Adimora, A. A., Joseph, S., Kuruc, J. D., Gay, C. L., Margolis, D. M., Archin, N., Brumme, Z. L., Swanstrom, R., & Goonetilleke, N. (2020). The HIV-1 latent reservoir is largely sensitive to circulating T cells. ELife, 9, e57246. https://doi.org/10.7554/eLife.57246 Cite
HUVECs or human coronary artery endothelial cells were pre-treated with the antiretrovirals abacavir sulphate, tenofovir disoproxil fumarate or tenofovir alafenamide.
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Montefiore Health System and Albert Einstein College of Medicine have received a five-year, $111 million grant from the National Cancer Institute to lead this research consortium.