[British Journal of Nutrition] Researchers investigated the role and underlying molecular mechanism of Tau on protein synthesis and proliferation of C2C12 myoblast cells.
[Circulation Journal] The expression of nuclear paraspeckle assembly transcript 1, miR-30d-5p and A disintegrin and metalloprotease 10 was measured by qRT-PCR and western blot. Functional experiments were conducted by using a CCK-8 assay, EDU assay, flow cytometry, western blot, ELISA, and commercial kits.
[Bioengineered] The underlying molecular mechanisms of PSP in improving insulin resistance (IR) in skeletal muscle remain unclear. PA induced an IR model in L6 myotubes. After treatment, cell proliferation was measured using the CCK8.