embryonic stem cells

[Cell Reports] The authors identified O-GlcNAcylation of proteasome activator subunit 3 (Psme3) protein as a node of the ESC pluripotency network. Mechanistically, O-GlcNAc modification of serine 111 (S111) of Psme3 promoted degradation of Ddx6, which was essential for processing body assembly, resulting in the maintenance of ESC pluripotent state.

[Journal of Cell Science] Human umbilical vein endothelial cells (HUVECs) elongated, activated TAGLN promoter and increased TAGLN transcripts in angiogenesis model. Genetic disruption of TAGLN augmented angiogenic behaviors of HUVECs, as did the disruption of TAGLN2 and TAGLN3 genes.

[Scientific Reports] To study of the relationship between DMR methylation and imprinted gene expression, scientists differentiated wild-type and Zfp57-/- hybrid mouse ESCs into neural precursor cells and evaluated allelic expression of imprinted genes.

[Cell Communication and Signaling] JMJD3 has been found to enhance self-renewal ability and reduce the differentiation capacity of ESCs and multipotent stem cells. The authors discuss the recent advances of JMJD3 function in stem cell fate.

[Scientific Reports] Scientists investigated whether co-culture of ESCs with HEI-OC1 cells promoted differentiation. Dissociated or embryonic bodies of ESCs were introduced to a conditioned and inactivated confluent layer of HEI-OC1 cells for 14 days.

[Cell Reports] The authors investigated mutant HTT-associated changes in gene expression by mouse and human striatal astrocytes, as well as in mouse microglia, to identify commonalities in glial pathobiology across species and models.