Site-Specific O-GlcNAcylation of Psme3 Maintains Mouse Stem Cell Pluripotency by Impairing P-Body Homeostasis

The authors identified O-GlcNAcylation of proteasome activator subunit 3 (Psme3) protein as a node of the ESC pluripotency network. Mechanistically, O-GlcNAc modification of serine 111 (S111) of Psme3 promoted degradation of Ddx6, which was essential for processing body assembly, resulting in the maintenance of ESC pluripotent state.