embryonic stem cells

[Stem Cell Research & Therapy] Researchers constructed a stemness index for single-cell samples (StemSC) based on relative expression orderings of gene pairs.

[Nature Communications] The authors established DamID- and HiC-based methodologies to generate accurate genome-wide maps of nucleolar associated domains in ESCs and neural progenitor cells, revealing layers of genome compartmentalization with distinct, repressive chromatin states based on the interaction with the nucleolus, nuclear lamina, or both.

[Metabolic Engineering] By screening a fluorescent probe library, investigators found that intracellular glycogen led to specific reactivity to CDg4, a glycogen fluorescence sensor, in both human and mouse naïve ESCs.

[Cell Reports] By targeting 1,700 transcription factors with CRISPR loss-of-function screen, researchers found that ZBTB11 and ZFP131 were required for ESC pluripotency.

[Journal of Cell Science] Researchers reported that SIRT1, the most conserved NAD+-dependent protein deacetylase highly expressed in perinatal heart, played an important role in regulating cardiomyocyte remodeling during development.

[Glycoconjugate Journal] The authors indicate that GD3 synthase contributes to gefitinib resistance in epidermal growth factor receptor-positive breast cancer cells, and is a potentially useful therapeutic target in drug-resistant breast cancers.