Researchers developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable human intestinal epithelial cells (hIECs) progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4.
6445212 RZBTHVWT items 1 apa default asc 1
Kwon, O., Jung, K. B., Lee, K.-R., Son, Y. S., Lee, H., Kim, J.-J., Kim, K., Lee, S., Song, Y.-K., Jung, J., Park, K., Kim, D.-S., Son, M. J., Lee, M.-O., Han, T.-S., Cho, H.-S., Oh, S. J., Chung, H., Kim, S.-H., … Son, M.-Y. (2021). The development of a functional human small intestinal epithelium model for drug absorption. Science Advances, 7(23), eabh1586. https://doi.org/10.1126/sciadv.abh1586 Cite
Using mouse mammary tissue ex vivo, scientists showed that testosterone-related progestins induced the progesterone receptor (PR) target and mediator of PR signaling-induced cell proliferation receptor activator of NF-κB ligand, whereas progestins with anti-androgenic properties in reporter assays did not.
[EMBO Molecular Medicine]
6445212 DFYT47KD items 1 apa default asc 1
Scientists studied the biological function of cytoplasmic maspin in breast cancer cell lines. Subcellular localization of maspin in MDA-MB-231 breast cancer cells was mainly detected in the cytoplasm, whereas in MCF10A mammary epithelial cells, maspin was present in both cytoplasm and nucleus.
6445212 N3XKXTWK items 1 apa default asc 1
Researchers analyzed colonic tissue samples from patients with ulcerative colitis (UC) and constructed specific intestinal epithelial phenazine biosynthesis-like domain-containing protein (PBLD)-deficient mice to investigate the role of this protein in UC pathogenesis.
[Cell Death & Disease]
6445212 EPQ3I58H items 1 apa default asc 1
The clustering of integrin α5ß1 at the plasma membrane of colorectal cancer-derived epithelial cells modulated their ability to respond to stimulation by receptor tyrosine kinase-activating growth factors EGF, NRG and HGF, through GSK3-mediated suppression of Akt pathway.
6445212 HTDFTD5G items 1 apa default asc 1
Scientists examined the relationship of RAB11A to epithelial junctional complexes, YAP, and the associated consequences on colonic epithelial tissue repair.
[Journal of Biological Chemistry]
6445212 QB2RGLIN items 1 apa default asc 1
Cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) deficiency led to intestinal stem cell loss and compromised intestinal barrier integrity upon dextran sodium sulfate-induced acute injury.
[Proceedings of the National Academy of Sciences of the United States of America]
6445212 WMV73XQ6 items 1 apa default asc 1
Scientists developed a live cell ratiometric fluorescent Optical Stem Cell Activity Reporter (OSCAR). Using the small intestinal crypt as a model, OSCAR revealed in real time the dynamics of dormancy induction and cellular differentiation in vitro, and allowed for the identification and isolation of several populations of transcriptionally diverse OSCARhigh and OSCARlow intestinal epithelial cell states in vivo.
6445212 3U8KUSAM items 1 apa default asc 1
Investigators generated CYP3A4-expressing Caco-2 cells and attempted to establish a model that could simultaneously evaluate drug absorption and metabolism.
6445212 L8AA9N4Q items 1 apa default asc 1
The authors review recent advances within intestinal regenerative biology and the emerging concept of fetal-like reprogramming, in which the adult intestinal epithelium transiently enters a repair-associated state reminiscent of ontologically pre-existing stages.
[Current Opinion in Genetics & Development]
6445212 SB6E55S3 items 1 apa default asc 1
Scientists review the biology of Inositol-requiring enzyme 1β (IRE1β), an ER-resident endonuclease and paralogue of the most evolutionarily conserved ER stress sensor IRE1α.
6445212 3979VR4P items 1 apa default asc 1