Enhancing Myocardial Repair with CardioClusters

Mesenchymal stem cells, endothelial progenitor cells and c-Kit+ cardiac interstitial cells when cultured together spontaneously formed scaffold-free 3D microenvironments termed CardioClusters.
[Nature Communications]
Monsanto, M. M., Wang, B. J., Ehrenberg, Z. R., Echeagaray, O., White, K. S., Alvarez, R., Fisher, K., Sengphanith, S., Muliono, A., Gude, N. A., & Sussman, M. A. (2020). Enhancing myocardial repair with CardioClusters. Nature Communications, 11(1), 3955. https://doi.org/10.1038/s41467-020-17742-z Cite
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Distinct Phenotypes of Cancer Cells on Tissue Matrix Gel

Scientists investigated mammary epithelial cell phenotypes and metabolic profiles on animal breast extracellular matrix-derived tissue matrix gel, Col I, and Matrigel. Atomic force microscopy, fluorescence microscopy, acini formation assay, differentiation experiments, spatial migration/invasion assays, proliferation assay, and nuclear magnetic resonance spectroscopy were used to examine biological phenotypes and metabolic changes.
[Breast Cancer Research]
Distinct phenotypes of cancer cells on tissue matrix gel | Breast Cancer Research | Full Text. (n.d.). Retrieved August 6, 2020, from https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-020-01321-7 Cite
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Functional Characterization of Three-Dimensional Cortical Cultures for In Vitro Modeling of Brain Networks

Scientists present an experimental system based on 3D scaffold-based cortical neuron cultures in which they expressed the genetically encoded calcium indicator GCaMP6f to record neuronal activity at the millimeter-scale.
[iScience]
Functional characterization of three-dimensional cortical cultures for in vitro modeling of brain networks: iScience. (n.d.). Retrieved August 6, 2020, from https://www.cell.com/iscience/fulltext/S2589-0042(20)30624-6 Cite
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Intercellular Communication Controls Agonist-Induced Calcium Oscillations Independently of Gap Junctions in Smooth Muscle Cells

Investigators report the existence of a communication system among human smooth muscle cells that used mechanical forces to frequency modulate long-range calcium waves.
[Science Advances]
Stasiak, S. E., Jamieson, R. R., Bouffard, J., Cram, E. J., & Parameswaran, H. (2020). Intercellular communication controls agonist-induced calcium oscillations independently of gap junctions in smooth muscle cells. Science Advances, 6(32), eaba1149. https://doi.org/10.1126/sciadv.aba1149 Cite
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Pancreatic Ductal Adenocarcinoma Progression Is Restrained by Stromal Matrix

Experimentally manipulating stromal matrix with an anti– lysyl oxidase like-2 antibody in syngeneic orthotopic pancreatic ductal adenocarcinoma mouse models significantly decreased matrix content, led to lower tissue stiffness, lower contrast retention on computed tomography, and accelerated tumor growth, resulting in diminished overall survival.
[Journal of Clinical Investigation]
Jiang, H., Torphy, R. J., Steiger, K., Hongo, H., Ritchie, A. J., Kriegsmann, M., Horst, D., Umetsu, S. E., Joseph, N. M., McGregor, K., Pishvaian, M. J., Blais, E. M., Lu, B., Li, M., Hollingsworth, M., Stashko, C., Volmar, K., Yeh, J. J., Weaver, V. M., … Collisson, E. A. (2020). Pancreatic ductal adenocarcinoma progression is restrained by stromal matrix. The Journal of Clinical Investigation, 130(9). https://doi.org/10.1172/JCI136760 Cite
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Amyloid Aggregates Accumulate in Melanoma Metastasis Modulating YAP Activity

Using secretome analyses, the authors revealed the extracellular accumulation of amyloidogenic proteins, i.e. premelanosome protein, in metastatic melanoma, together with proteins that assist amyloid maturation into fibrils.
[EMBO Reports]
Matafora, V., Farris, F., Restuccia, U., Tamburri, S., Martano, G., Bernardelli, C., Sofia, A., Pisati, F., Casagrande, F., Lazzari, L., Marsoni, S., Bonoldi, E., & Bachi, A. (2020). Amyloid aggregates accumulate in melanoma metastasis modulating YAP activity. EMBO Reports, n/a(n/a), e50446. https://doi.org/10.15252/embr.202050446 Cite
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Osteogenic and Adipogenic Differentiation of Mesenchymal Stem Cells in Gelatin Solutions of Different Viscosities

A biphasic gelatin solution/hydrogel system was used for 3D culture of human bone marrow–derived mesenchymal stem cells to investigate the influence of gelatin solution viscosity on simultaneous osteogenic and adipogenic differentiation at the same culture condition.
[Advanced Healthcare Materials]
Lee, K., Chen, Y., Yoshitomi, T., Kawazoe, N., Yang, Y., & Chen, G. (n.d.). Osteogenic and Adipogenic Differentiation of Mesenchymal Stem Cells in Gelatin Solutions of Different Viscosities. Advanced Healthcare Materials, n/a(n/a), 2000617. https://doi.org/10.1002/adhm.202000617 Cite
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Formation and Optimization of Three-Dimensional Organoids Generated From Urine-Derived Stem Cells for Renal Function In Vitro

Researchers aimed to develop a novel kind of 3D organoids generated from urine-derived stem cells and to explore whether kidney-specific extracellular matrix could enable such organoids for renal function in vitro.
[Stem Cell Research & Therapy]
Sun, G., Ding, B., Wan, M., Chen, L., Jackson, J., & Atala, A. (2020). Formation and optimization of three-dimensional organoids generated from urine-derived stem cells for renal function in vitro. Stem Cell Research & Therapy, 11(1), 309. https://doi.org/10.1186/s13287-020-01822-4 Cite
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The Mesenchymal Context in Inflammation, Immunity and Cancer

Investigators provide a concise overview of the contextual contribution of fibroblastic mesenchymal cells in physiology and disease and bring into focus emerging evidence for both their heterogeneity at the single-cell level and their tissue-specific, spatiotemporal functional diversity.
[Nature Immunology]
Koliaraki, V., Prados, A., Armaka, M., & Kollias, G. (2020). The mesenchymal context in inflammation, immunity and cancer. Nature Immunology, 1–9. https://doi.org/10.1038/s41590-020-0741-2 Cite
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Fabrication of Homotypic Neural Ribbons as a Multiplex Platform Optimized for Spinal Cord Delivery

Neural ribbons offer multifunctionality in vitro including co-encapsulation of the injury site ESM modifier chondroitinase ABC, tested in glial scar models, and ability of cervically-patterned spinal cord neural stem cells to differentiate within neural ribbons and project axons for integration with 3D external matrices.
[Scientific Reports]
Olmsted, Z. T., Stigliano, C., Badri, A., Zhang, F., Williams, A., Koffas, M. A. G., Xie, Y., Linhardt, R. J., Cibelli, J., Horner, P. J., & Paluh, J. L. (2020). Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery. Scientific Reports, 10(1), 12939. https://doi.org/10.1038/s41598-020-69274-7 Cite
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Structure of a Collagen VI α3 Chain VWA Domain Array: Adaptability and Functional Implications of Myopathy Causing Mutations

Collagen VI is a ubiquitous heterotrimeric protein of the ECM that plays an essential role in the proper maintenance of skeletal muscle. Scientists investigated structure-function relationships of N2 domain mutations to shed light on their possible myopathy mechanisms.
[Journal of Biological Chemistry]
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Pan-Cancer Analysis of the Genomic Alterations and Mutations of the Matrisome

Mining The Cancer Genome Atlas data, scientists found that copy number alterations and mutations are frequent in matrisome genes, even more so than in the rest of the genome.
[Cancers]
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