Fibronectin Fibril Alignment Is Established upon Initiation of Extracellular Matrix Assembly

Researchers investigated the initiation of fibronectin (FN) matrix assembly using fibroblasts that assemble parallel extracellular matrix fibrils and found that matrix assembly sites, where FN fibrillogenesis is initiated, were oriented in parallel at the cell poles.
[Molecular Biology of the Cell]
Garrison, C. M., & Schwarzbauer, J. E. (2021). Fibronectin fibril alignment is established upon initiation of extracellular matrix assembly. Molecular Biology of the Cell, mbc.E20-08-0533. https://doi.org/10.1091/mbc.E20-08-0533 Cite
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Accelerated In Vitro Recellularization of Decellularized Porcine Pericardium for Cardiovascular Grafts

Decellularized porcine pericardium scaffolds were modified by growing a fibrin mesh on the surface and inside the scaffolds, and by attaching heparin and human vascular endothelial growth factor to this mesh. Then the scaffolds were seeded with human adipose tissue-derived stem cells.
[Biomedical Materials]
Filova, E., Steinerova, M., Travnickova, M., Knitlova, J., Musilkova, J., Eckhardt, A., Hadraba, D., Matejka, R., Prazak, S., Stepanovska, J., Kucerova, J., Riedel, T., Brynda, E., Lodererova, A., Honsova, E., Pirk, J., Konarik, M., & Bacakova, L. (2021). Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts. Biomedical Materials, 16(2), 025024. https://doi.org/10.1088/1748-605x/abbdbd Cite
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Nerve Growth Factor Receptor Increases the Tumor Growth and Metastatic Potential of Triple-Negative Breast Cancer Cells

By using RNA-seq analysis, the authors found that nerve growth factor receptor was highly expressed in metastatic lung clones of MDA-MB-231 cells.
[Oncogene]
Wu, R., Li, K., Yuan, M., & Luo, K. Q. (2021). Nerve growth factor receptor increases the tumor growth and metastatic potential of triple-negative breast cancer cells. Oncogene, 1–17. https://doi.org/10.1038/s41388-021-01691-y Cite
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3D Spheroid Models of Pediatric SHH Medulloblastoma Mimic Tumor Biology, Drug Response and Metastatic Dissemination

When grown under the same stem cell enriching conditions, SHH subgroup medulloblastoma cell lines established tight, highly reproducible 3D spheroids that could be maintained for weeks in culture and formed pathophysiological oxygen gradients.
[Scientific Reports]
Roper, S. J., Linke, F., Scotting, P. J., & Coyle, B. (2021). 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination. Scientific Reports, 11(1), 4259. https://doi.org/10.1038/s41598-021-83809-6 Cite
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Inhibition of miR-135a-5p Attenuates Vascular Smooth Muscle Cell Proliferation and Vascular Remodeling in Hypertensive Rats

Scientists determined the roles and mechanisms of miR-135a-5p intervention in attenuating VSMC proliferation and vascular remodeling in spontaneously hypertensive rats
[Acta Pharmacologica Sinica]
Ye, C., Tong, Y., Wu, N., Wan, G., Zheng, F., Chen, J., Lei, J., Zhou, H., Chen, A., Wang, J., Chen, Q., Li, Y., Kang, Y., & Zhu, G. (2021). Inhibition of miR-135a-5p attenuates vascular smooth muscle cell proliferation and vascular remodeling in hypertensive rats. Acta Pharmacologica Sinica, 1–10. https://doi.org/10.1038/s41401-020-00608-x Cite
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Prostaglandin F2α and EP2 Agonists, and a ROCK Inhibitor Modulate the Formation of 3D Organoids of Grave’s Orbitopathy Related Human Orbital Fibroblasts

3D organoids were prepared from Grave’s orbitopathy (GO) related human orbital fibroblasts obtained from patients with GO.
[Experimental Eye Research]
Ichioka, H., Ida, Y., Watanabe, M., Ohguro, H., & Hikage, F. (2021). Prostaglandin F2α and EP2 agonists, and a ROCK inhibitor modulate the formation of 3D organoids of Grave’s orbitopathy related human orbital fibroblasts. Experimental Eye Research, 108489. https://doi.org/10.1016/j.exer.2021.108489 Cite
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The Molecular Conformation of Silk Fibroin Regulates Osteogenic Cell Behavior by Modulating the Stability of the Adsorbed Protein-Material Interface

Osteogenic differentiation of bone marrow (BM)-MSCs was stimulated on high β-sheet substrates. Investigators indicated that the β-sheet content influences osteogenic differentiation of BMSCs on silk fibroin materials in vitro by modulating the stability of the adsorbed protein-material interface, which proceeds via protein-focal adhesion-cytoskeleton links and subsequent intracellular mechanotransduction.
[Bone Research]
Long, Y., Cheng, X., Jansen, J. A., Leeuwenburgh, S. G. C., Mao, J., Yang, F., & Chen, L. (2021). The molecular conformation of silk fibroin regulates osteogenic cell behavior by modulating the stability of the adsorbed protein-material interface. Bone Research, 9(1), 1–12. https://doi.org/10.1038/s41413-020-00130-0 Cite
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The Lectin-Like Domain of Thrombomodulin Inhibits β1 Integrin-Dependent Binding of Human Breast Cancer-Derived Cell Lines to Fibronectin

Using a V-well cell adhesion assay or flow cytometry analysis with fluorescent beads, the authors found that both TM domain 12-Fc and TM domain 23-Fc inhibited the binding between β1 integrin of human breast cancer-derived cell lines and fibronectin.
[Biomedicines]
Kawamoto, E., Nago, N., Okamoto, T., Gaowa, A., Masui-Ito, A., Akama, Y., Darkwah, S., Appiah, M. G., Myint, P. K., Obeng, G., Ito, A., Caidengbate, S., Esumi, R., Yamaguchi, T., Park, E. J., Imai, H., & Shimaoka, M. (2021). The Lectin-Like Domain of Thrombomodulin Inhibits β1 Integrin-Dependent Binding of Human Breast Cancer-Derived Cell Lines to Fibronectin. Biomedicines, 9(2), 162. https://doi.org/10.3390/biomedicines9020162 Cite
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Reduced Pericyte and Tight Junction Coverage in Old Diabetic Rats Are Associated with Hyperglycemia-Induced Cerebrovascular Pericyte Dysfunction

Using a nonobese T2DN diabetes mellitus (DM) rat, researchers isolated parenchymal arterioles, cultured cerebral microvascular pericytes, and examined whether cerebrovascular pericyte in DM was damaged and whether pericyte dysfunction may play a role in the regulation of cerebral hemodynamics and blood-brain barrier integrity.
[American Journal of Physiology-Heart and Circulatory Physiology]
Liu, Y., Zhang, H., Wang, S., Guo, Y., Fang, X., Zheng, B., Gao, W., Yu, H., Chen, Z., Roman, R. J., & Fan, F. (2020). Reduced pericyte and tight junction coverage in old diabetic rats are associated with hyperglycemia-induced cerebrovascular pericyte dysfunction. American Journal of Physiology-Heart and Circulatory Physiology, 320(2), H549–H562. https://doi.org/10.1152/ajpheart.00726.2020 Cite
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Activated Intestinal Muscle Cells Promote Preadipocyte Migration: A Novel Mechanism for Creeping Fat Formation in Crohn’s Disease

Crohn’s disease muscularis propria cells produced an ECM scaffold which was in direct spatial and functional contact with the immediately overlaid creeping fat.
[Gut]
Mao, R., Doyon, G., Gordon, I. O., Li, J., Lin, S., Wang, J., Le, T. H. N., Elias, M., Kurada, S., Southern, B., Olman, M., Chen, M., Zhao, S., Dejanovic, D., Chandra, J., Mukherjee, P. K., West, G., Wagoner, D. R. V., Fiocchi, C., & Rieder, F. (2021). Activated intestinal muscle cells promote preadipocyte migration: a novel mechanism for creeping fat formation in Crohn’s disease. Gut. https://doi.org/10.1136/gutjnl-2020-323719 Cite
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Non Pharmacological High-Intensity Ultrasound Treatment of Human Dermal Fibroblasts to Accelerate Wound Healing

The authors investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprised of human dermal fibroblasts.
[Scientific Reports]
Lee, J. Y., Min, D.-J., Kim, W., Bin, B.-H., Kim, K., & Cho, E.-G. (2021). Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing. Scientific Reports, 11(1), 2465. https://doi.org/10.1038/s41598-021-81878-1 Cite
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