Human hematopoietic stem cells were differentiated into megakaryocytes, enabling the transfection of miR-204-5p and the recovery of subsequent platelet-like structures.
[Thrombosis and Haemostasis]
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Researchers outline a simple new technique of isolating mouse BMEC primary culture and a suitable method to evaluate the function and dysregulation of BMEC in in vitro studies using mouse models.
[Stem Cell Research & Therapy]
Investigators demonstrated that T cell phenotypic signatures associate with viral reservoir size in a cohort of HIV vertically infected children and young adults under durable viral control, and who initiated anti-retroviral therapy under two years old.
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Rinaldi, S., Armas, L. de, Dominguez-Rodríguez, S., Pallikkuth, S., Dinh, V., Pan, L., Gӓrtner, K., Pahwa, R., Cotugno, N., Rojo, P., Nastouli, E., Klein, N., Foster, C., Rossi, A. D., Giaquinto, C., Rossi, P., Palma, P., & Consortium, on behalf of E. (2021). T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals. PLOS Pathogens, 17(4), e1009533. https://doi.org/10.1371/journal.ppat.1009533 Cite
Investigators assessed the magnitude, function and phenotype of SARS-CoV-2-specific CD4 T cells in 95 hospitalized COVID-19 patients and 38 non-COVID-19 patients, using flow cytometry.
[Journal of Clinical Investigation]
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Riou, C., Bruyn, E. D., Stek, C., Daroowala, R., Goliath, R. T., Abrahams, F., Said-Hartley, Q., Allwood, B. W., Hsiao, N.-Y., Wilkinson, K. A., Arlehamn, C. S. L., Sette, A., Wasserman, S., & Wilkinson, R. J. (2021). Relationship of SARS-CoV-2-specific CD4 response to COVID-19 severity and impact of HIV-1 and Tuberculosis co-infection. The Journal of Clinical Investigation. https://doi.org/10.1172/JCI149125 Cite
Scientists screened all ten asparagine-linked glycosylation (ALG) members in breast cancer patients’ samples by RT-PCR. Cancer stem-like traits were assessed by RT-PCR, Western blot, and flow cytometry.
[Journal of Experimental & Clinical Cancer Research]
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Sun, X., He, Z., Guo, L., Wang, C., Lin, C., Ye, L., Wang, X., Li, Y., Yang, M., Liu, S., Hua, X., Wen, W., Lin, C., Long, Z., Zhang, W., Li, H., Jian, Y., Zhu, Z., Wu, X., & Lin, H. (2021). ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer. Journal of Experimental & Clinical Cancer Research, 40(1), 149. https://doi.org/10.1186/s13046-021-01932-8 Cite
A cord blood product was analyzed by three methods for cell viability, two methods for assessment of cell morphology and cell type, multicolor flow cytometry to identify stem cells, and enzyme-linked immunosorbent assay.
[The American Journal of Sports Medicine]
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Fortier, L. A., Cercone, M., Keller, L. E., Delco, M. L., Becktell, L., & Wells, K. V. (2021). Amnion and Umbilical Cord–Derived Products in Sports Medicine: From Basic Science to Clinical Application. The American Journal of Sports Medicine, 03635465211010466. https://doi.org/10.1177/03635465211010466 Cite
INS-1 cells were treated with autoantibody against the angiotensin II type I receptor, and cell viability, apoptosis, and autophagy-related proteins were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and western blot analysis, respectively.
[Acta Biochimica et Biophysica Sinica]
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Researchers suggest that KCNQ1OT1 and its target gene miR-181a-5p regulated myocardial inflammation and cardiomyocyte apoptosis by modulating PDCD4 in diabetic cardiomyopathy.
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Long non-coding RNA KCNQ1OT1 increases the expression of PDCD4 by targeting miR-181a-5p, contributing to cardiomyocyte apoptosis in diabetic cardiomyopathy | SpringerLink. (n.d.). Retrieved May 3, 2021, from https://link.springer.com/article/10.1007%2Fs00592-021-01713-x Cite
Motor function was analyzed by three behavioral tests. Engraftment of human amniotic fluid stem cell in organs were assessed by flow cytometry and RNA scope.
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Shaw, S. W., Peng, S.-Y., Liang, C.-C., Lin, T.-Y., Cheng, P.-J., Hsieh, T.-T., Chuang, H.-Y., De Coppi, P., & David, A. L. (2021). Prenatal transplantation of human amniotic fluid stem cell could improve clinical outcome of type III spinal muscular atrophy in mice. Scientific Reports, 11(1), 9158. https://doi.org/10.1038/s41598-021-88559-z Cite
Investigators present a high throughput strategy for identifying and isolating megakaryocytes and their progenitor cells from a heterogeneous population of bone marrow samples.
Researchers suggested that the increase in glucose metabolism promotes cardiac regeneration in neonatal mouse heart.
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Fajardo, V. M., Feng, I., Chen, B. Y., Perez-Ramirez, C. A., Shi, B., Clark, P., Tian, R., Lien, C.-L., Pellegrini, M., Christofk, H., Nakano, H., & Nakano, A. (2021). GLUT1 overexpression enhances glucose metabolism and promotes neonatal heart regeneration. Scientific Reports, 11(1), 8669. https://doi.org/10.1038/s41598-021-88159-x Cite