miR-204-5p and Platelet Function Regulation: Insight into a Mechanism Mediated by CDC42 and GPIIbIIIa

Human hematopoietic stem cells were differentiated into megakaryocytes, enabling the transfection of miR-204-5p and the recovery of subsequent platelet-like structures.
[Thrombosis and Haemostasis]
Garcia, A., Dunoyer-Geindre, S., Nolli, S., Strassel, C., Reny, J.-L., & Fontana, P. (2021). miR-204-5p and platelet function regulation: insight into a mechanism mediated by CDC42 and GPIIbIIIa. Thrombosis and Haemostasis, 0(AAM). https://doi.org/10.1055/a-1497-9649 Cite
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A Novel Strategy for Isolation of Mice Bone Marrow Endothelial Cells (BMECs)

Researchers outline a simple new technique of isolating mouse BMEC primary culture and a suitable method to evaluate the function and dysregulation of BMEC in in vitro studies using mouse models.
[Stem Cell Research & Therapy]
Smith, A. O., Adzraku, S. Y., Ju, W., Qiao, J., Xu, K., & Zeng, L. (2021). A novel strategy for isolation of mice bone marrow endothelial cells (BMECs). Stem Cell Research & Therapy, 12(1), 267. https://doi.org/10.1186/s13287-021-02352-3 Cite
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T Cell Immune Discriminants of HIV Reservoir Size in a Pediatric Cohort of Perinatally Infected Individuals

Investigators demonstrated that T cell phenotypic signatures associate with viral reservoir size in a cohort of HIV vertically infected children and young adults under durable viral control, and who initiated anti-retroviral therapy under two years old.
[PLoS Pathogens]
Rinaldi, S., Armas, L. de, Dominguez-Rodríguez, S., Pallikkuth, S., Dinh, V., Pan, L., Gӓrtner, K., Pahwa, R., Cotugno, N., Rojo, P., Nastouli, E., Klein, N., Foster, C., Rossi, A. D., Giaquinto, C., Rossi, P., Palma, P., & Consortium, on behalf of E. (2021). T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals. PLOS Pathogens, 17(4), e1009533. https://doi.org/10.1371/journal.ppat.1009533 Cite
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Relationship of SARS-CoV-2-Specific CD4 Response to COVID-19 Severity and Impact of HIV-1 and Tuberculosis Co-Infection

Investigators assessed the magnitude, function and phenotype of SARS-CoV-2-specific CD4 T cells in 95 hospitalized COVID-19 patients and 38 non-COVID-19 patients, using flow cytometry.
[Journal of Clinical Investigation]
Riou, C., Bruyn, E. D., Stek, C., Daroowala, R., Goliath, R. T., Abrahams, F., Said-Hartley, Q., Allwood, B. W., Hsiao, N.-Y., Wilkinson, K. A., Arlehamn, C. S. L., Sette, A., Wasserman, S., & Wilkinson, R. J. (2021). Relationship of SARS-CoV-2-specific CD4 response to COVID-19 severity and impact of HIV-1 and Tuberculosis co-infection. The Journal of Clinical Investigation. https://doi.org/10.1172/JCI149125 Cite
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ALG3 Contributes to Stemness and Radioresistance through Regulating Glycosylation of TGF-β Receptor II in Breast Cancer

Scientists screened all ten asparagine-linked glycosylation (ALG) members in breast cancer patients’ samples by RT-PCR. Cancer stem-like traits were assessed by RT-PCR, Western blot, and flow cytometry.
[Journal of Experimental & Clinical Cancer Research]
Sun, X., He, Z., Guo, L., Wang, C., Lin, C., Ye, L., Wang, X., Li, Y., Yang, M., Liu, S., Hua, X., Wen, W., Lin, C., Long, Z., Zhang, W., Li, H., Jian, Y., Zhu, Z., Wu, X., & Lin, H. (2021). ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer. Journal of Experimental & Clinical Cancer Research, 40(1), 149. https://doi.org/10.1186/s13046-021-01932-8 Cite
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Amnion and Umbilical Cord–Derived Products in Sports Medicine: From Basic Science to Clinical Application

A cord blood product was analyzed by three methods for cell viability, two methods for assessment of cell morphology and cell type, multicolor flow cytometry to identify stem cells, and enzyme-linked immunosorbent assay.
[The American Journal of Sports Medicine]
Fortier, L. A., Cercone, M., Keller, L. E., Delco, M. L., Becktell, L., & Wells, K. V. (2021). Amnion and Umbilical Cord–Derived Products in Sports Medicine: From Basic Science to Clinical Application. The American Journal of Sports Medicine, 03635465211010466. https://doi.org/10.1177/03635465211010466 Cite
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Autoantibody against Angiotensin II Type I Receptor Induces Pancreatic β-Cell Apoptosis via Enhancing Autophagy

INS-1 cells were treated with autoantibody against the angiotensin II type I receptor, and cell viability, apoptosis, and autophagy-related proteins were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and western blot analysis, respectively.
[Acta Biochimica et Biophysica Sinica]
Wang, J., Li, D., Zhang, Z., Zhang, Y., Lei, Z., Jin, W., Cao, J., & Jiao, X. (2021). Autoantibody against angiotensin II type I receptor induces pancreatic β-cell apoptosis via enhancing autophagy. Acta Biochimica et Biophysica Sinica, gmab049. https://doi.org/10.1093/abbs/gmab049 Cite
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Long Non-Coding RNA KCNQ1OT1 Increases the Expression of PDCD4 by Targeting miR-181a-5p, Contributing to Cardiomyocyte Apoptosis in Diabetic Cardiomyopathy

Researchers suggest that KCNQ1OT1 and its target gene miR-181a-5p regulated myocardial inflammation and cardiomyocyte apoptosis by modulating PDCD4 in diabetic cardiomyopathy.
[Acta Diabetologica]
Long non-coding RNA KCNQ1OT1 increases the expression of PDCD4 by targeting miR-181a-5p, contributing to cardiomyocyte apoptosis in diabetic cardiomyopathy | SpringerLink. (n.d.). Retrieved May 3, 2021, from https://link.springer.com/article/10.1007%2Fs00592-021-01713-x Cite
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Prenatal Transplantation of Human Amniotic Fluid Stem Cell Could Improve Clinical Outcome of Type III Spinal Muscular Atrophy in Mice

Motor function was analyzed by three behavioral tests. Engraftment of human amniotic fluid stem cell in organs were assessed by flow cytometry and RNA scope.
[Scientific Reports]
Shaw, S. W., Peng, S.-Y., Liang, C.-C., Lin, T.-Y., Cheng, P.-J., Hsieh, T.-T., Chuang, H.-Y., De Coppi, P., & David, A. L. (2021). Prenatal transplantation of human amniotic fluid stem cell could improve clinical outcome of type III spinal muscular atrophy in mice. Scientific Reports, 11(1), 9158. https://doi.org/10.1038/s41598-021-88559-z Cite
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High-Throughput Enrichment and Isolation of Megakaryocyte Progenitor Cells from the Mouse Bone Marrow

Investigators present a high throughput strategy for identifying and isolating megakaryocytes and their progenitor cells from a heterogeneous population of bone marrow samples.
[Scientific Reports]
Bush, L. M., Healy, C. P., Marvin, J. E., & Deans, T. L. (2021). High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow. Scientific Reports, 11(1), 8268. https://doi.org/10.1038/s41598-021-87681-2 Cite
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GLUT1 Overexpression Enhances Glucose Metabolism and Promotes Neonatal Heart Regeneration

Researchers suggested that the increase in glucose metabolism promotes cardiac regeneration in neonatal mouse heart.
[Scientific Reports]
Fajardo, V. M., Feng, I., Chen, B. Y., Perez-Ramirez, C. A., Shi, B., Clark, P., Tian, R., Lien, C.-L., Pellegrini, M., Christofk, H., Nakano, H., & Nakano, A. (2021). GLUT1 overexpression enhances glucose metabolism and promotes neonatal heart regeneration. Scientific Reports, 11(1), 8669. https://doi.org/10.1038/s41598-021-88159-x Cite
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