Scientists demonstrated the physiological relevance and loss-of-function mechanism of a novel Caveolin3 interactor in gene-edited human iPSC-cardiomyocytes.
1254621 C3YV2BNM items 1 apa default asc 1
Peper Jonas, Kownatzki-Danger Daniel, Weninger Gunnar, Seibertz Fitzwilliam, Pronto Julius Ryan, Sutanto Henry, Pacheu Grau David, Hindmarsh Robin, Brandenburg Sören, Kohl Tobias, Hasenfuβ Gerd, Gotthardt Michael, Rog-Zielinska Eva A, Wollnik Bernd, Rehling Peter, Urlaub Henning, Wegener Jörg W, Heijman Jordi, Voigt Niels, … Lehnart Stephan E. (n.d.). Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes. Circulation Research, 0(0). https://doi.org/10.1161/CIRCRESAHA.119.316547 Cite
The structural information of the insulin-like growth factor 2 receptor (IGF2R), an overexpressed protein on activated hepatic stellate cells, were used for an in silico screening of novel IGF2R-specific peptide ligands.
[Journal of Materials Chemistry B]
6445212 7PE6FJMB items 1 apa default asc 1
Weber, F., Casalini, T., Valentino, G., Brülisauer, L., Andreas, N., Koeberle, A., Kamradt, T., Contini, A., & Luciani, P. (2021). Targeting transdifferentiated hepatic stellate cells and monitoring the hepatic fibrogenic process by means of IGF2R-specific peptides designed in silico. Journal of Materials Chemistry B. https://doi.org/10.1039/D0TB02372H Cite
Scientists unravelled the down-stream pathways associated with mitochondrial complex II (CII) inhibition and compared with CI inhibition and the Manganese neurotoxicity. Genome-wide transcriptomics of N27 neuronal cells exposed to 3-nitropropionic acid, compared with 1-methyl-4-phenylpyridinium and Mn revealed varied transcriptomic profile.
6807162 ISB7GX9N items 1 apa default asc 1
Ranganayaki, S., Jamshidi, N., Aiyaz, M., Rashmi, S.-K., Gayathri, N., Harsha, P. K., Padmanabhan, B., & Srinivas Bharath, M. M. (2021). Inhibition of mitochondrial complex II in neuronal cells triggers unique pathways culminating in autophagy with implications for neurodegeneration. Scientific Reports, 11(1), 1483. https://doi.org/10.1038/s41598-020-79339-2 Cite
The authors performed an in silico study of ADAMTS1 and endothelial markers in human gliomas, providing the basis to further assess these molecules in several primary glioblastoma-initiating cells and established glioblastoma cells with the ability to acquire an endothelial-like phenotype.
6445212 ADAMTS1SupportsEndothelialPlasticityofGliobla items 1 apa default asc 1
The phenolic and terpenophenolic compounds were assayed in the water extract of the hemp variety Futura 75. Protective effects were also investigated in human fibroblast and keratinocytes and isolate mouse skin specimens, which were exposed to hydrogen peroxide and/or to the extract.
6807162 55PGS76F items 1 apa default asc 1
di Giacomo, V., Recinella, L., Chiavaroli, A., Orlando, G., Cataldi, A., Rapino, M., Di Valerio, V., Politi, M., Antolini, M. D., Acquaviva, A., Bacchin, F., Di Mascio, M., Leone, S., Brunetti, L., Menghini, L., Carradori, S., Zengin, G., Ak, G., & Ferrante, C. (2021). Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens. Antioxidants, 10(1), 44. https://doi.org/10.3390/antiox10010044 Cite
The authors demonstrated that Cas9-ribonucleoprotein-based genome editors can correct two distinct mutant alleles within a single human cell precisely. Gene-corrected cells in an iPSC model of Pompe disease expressed the corrected transcript from both corrected alleles, leading to enzymatic cross-correction of diseased cells.
6630899 4KIQ4CTD items 1 apa default asc 1
Carlson-Stevermer, J., Das, A., Abdeen, A. A., Fiflis, D., Grindel, B. I., Saxena, S., Akcan, T., Alam, T., Kletzien, H., Kohlenberg, L., Goedland, M., Dombroe, M. J., & Saha, K. (2020). Design of efficacious somatic cell genome editing strategies for recessive and polygenic diseases. Nature Communications, 11(1), 6277. https://doi.org/10.1038/s41467-020-20065-8 Cite
Researchers constructed engineered heart tissues from two lines of fast- and slow-relaxing human iPSC-derived cardiomyocytes. Cell tracking via fluorescent nanocrystals confirmed the presence of both cell populations in the 50/50 mixed tissues at the time of mechanical characterization.
[Archives of Biochemistry and Biophysics]
1254621 NF7F2FI3 items 1 apa default asc 1
Human CD24–/CD44+ breast cancer stem cells (BCSCs) were treated with andrographolide in vitro for 24 hours. The cytotoxic effect of andrographolide on BCSCs was compared to that on human mesenchymal stem cells.
6445212 CBBP8RUR items 1 apa default asc 1
Wanandi, S. I., Limanto, A., Yunita, E., Syahrani, R. A., Louisa, M., Wibowo, A. E., & Arumsari, S. (2020). In silico and in vitro studies on the anti-cancer activity of andrographolide targeting survivin in human breast cancer stem cells. PLOS ONE, 15(11), e0240020. https://doi.org/10.1371/journal.pone.0240020 Cite
Researchers analyzed five public scRNA-seq pancreas datasets and performed fluorescence in situ hybridization, Western blotting and immunolocalization for ACE2 with extensive reagent validation on normal human pancreatic tissues across the lifespan, as well as those from COVID-19 cases.
6445218 V8FWWMPS items 1 apa default asc 1
Kusmartseva, I., Wu, W., Syed, F., Heide, V. V. D., Jorgensen, M., Joseph, P., Tang, X., Candelario-Jalil, E., Yang, C., Nick, H., Harbert, J. L., Posgai, A. L., Paulsen, J. D., Lloyd, R., Cechin, S., Pugliese, A., Campbell-Thompson, M., Heide, R. S. V., Evans-Molina, C., … Atkinson, M. A. (2020). Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19. Cell Metabolism, 0(0). https://doi.org/10.1016/j.cmet.2020.11.005 Cite
The authors focused on deubiquitinating enzymes, which regulate protein stability through ubiquitin–proteasome systems, and identified 26S proteasome non-ATPase regulatory subunit 14 (PSMD14) as a molecule related to melanoma growth using siRNA library screening.
6807162 Z56FV94P items 1 apa default asc 1
Investigators focused on microRNA-200b/c-3p whose expression was abundant in intact epidermis, but dramatically decreased in skin wounds.
[Cell Death & Disease]
6807162 4TZRPVTF items 1 apa default asc 1
Tang, H., Wang, X., Zhang, M., Yan, Y., Huang, S., Ji, J., Xu, J., Zhang, Y., Cai, Y., Yang, B., Lan, W., Huang, M., & Zhang, L. (2020). MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling. Cell Death & Disease, 11(10), 1–17. https://doi.org/10.1038/s41419-020-03132-2 Cite