Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes

Scientists demonstrated the physiological relevance and loss-of-function mechanism of a novel Caveolin3 interactor in gene-edited human iPSC-cardiomyocytes.
[Circulation Research]
Peper Jonas, Kownatzki-Danger Daniel, Weninger Gunnar, Seibertz Fitzwilliam, Pronto Julius Ryan, Sutanto Henry, Pacheu Grau David, Hindmarsh Robin, Brandenburg Sören, Kohl Tobias, Hasenfuβ Gerd, Gotthardt Michael, Rog-Zielinska Eva A, Wollnik Bernd, Rehling Peter, Urlaub Henning, Wegener Jörg W, Heijman Jordi, Voigt Niels, … Lehnart Stephan E. (n.d.). Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes. Circulation Research, 0(0). https://doi.org/10.1161/CIRCRESAHA.119.316547 Cite
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Targeting Transdifferentiated Hepatic Stellate Cells and Monitoring the Hepatic Fibrogenic Process by Means of IGF2R-Specific Peptides Designed In Silico

The structural information of the insulin-like growth factor 2 receptor (IGF2R), an overexpressed protein on activated hepatic stellate cells, were used for an in silico screening of novel IGF2R-specific peptide ligands.
[Journal of Materials Chemistry B]
Weber, F., Casalini, T., Valentino, G., Brülisauer, L., Andreas, N., Koeberle, A., Kamradt, T., Contini, A., & Luciani, P. (2021). Targeting transdifferentiated hepatic stellate cells and monitoring the hepatic fibrogenic process by means of IGF2R-specific peptides designed in silico. Journal of Materials Chemistry B. https://doi.org/10.1039/D0TB02372H Cite
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Inhibition of Mitochondrial Complex II in Neuronal Cells Triggers Unique Pathways Culminating in Autophagy with Implications for Neurodegeneration

Scientists unravelled the down-stream pathways associated with mitochondrial complex II (CII) inhibition and compared with CI inhibition and the Manganese neurotoxicity. Genome-wide transcriptomics of N27 neuronal cells exposed to 3-nitropropionic acid, compared with 1-methyl-4-phenylpyridinium and Mn revealed varied transcriptomic profile.
[Scientific Reports]
Ranganayaki, S., Jamshidi, N., Aiyaz, M., Rashmi, S.-K., Gayathri, N., Harsha, P. K., Padmanabhan, B., & Srinivas Bharath, M. M. (2021). Inhibition of mitochondrial complex II in neuronal cells triggers unique pathways culminating in autophagy with implications for neurodegeneration. Scientific Reports, 11(1), 1483. https://doi.org/10.1038/s41598-020-79339-2 Cite
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ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

The authors performed an in silico study of ADAMTS1 and endothelial markers in human gliomas, providing the basis to further assess these molecules in several primary glioblastoma-initiating cells and established glioblastoma cells with the ability to acquire an endothelial-like phenotype.
[Biomolecules]
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Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens

The phenolic and terpenophenolic compounds were assayed in the water extract of the hemp variety Futura 75. Protective effects were also investigated in human fibroblast and keratinocytes and isolate mouse skin specimens, which were exposed to hydrogen peroxide and/or to the extract.
[Antioxidants]
di Giacomo, V., Recinella, L., Chiavaroli, A., Orlando, G., Cataldi, A., Rapino, M., Di Valerio, V., Politi, M., Antolini, M. D., Acquaviva, A., Bacchin, F., Di Mascio, M., Leone, S., Brunetti, L., Menghini, L., Carradori, S., Zengin, G., Ak, G., & Ferrante, C. (2021). Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens. Antioxidants, 10(1), 44. https://doi.org/10.3390/antiox10010044 Cite
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Design of Efficacious Somatic Cell Genome Editing Strategies for Recessive and Polygenic Diseases

The authors demonstrated that Cas9-ribonucleoprotein-based genome editors can correct two distinct mutant alleles within a single human cell precisely. Gene-corrected cells in an iPSC model of Pompe disease expressed the corrected transcript from both corrected alleles, leading to enzymatic cross-correction of diseased cells.
[Nature Communications]
Carlson-Stevermer, J., Das, A., Abdeen, A. A., Fiflis, D., Grindel, B. I., Saxena, S., Akcan, T., Alam, T., Kletzien, H., Kohlenberg, L., Goedland, M., Dombroe, M. J., & Saha, K. (2020). Design of efficacious somatic cell genome editing strategies for recessive and polygenic diseases. Nature Communications, 11(1), 6277. https://doi.org/10.1038/s41467-020-20065-8 Cite
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Fast-Relaxing Cardiomyocytes Exert a Dominant Role in the Relaxation Behavior of Heterogeneous Myocardium

Researchers constructed engineered heart tissues from two lines of fast- and slow-relaxing human iPSC-derived cardiomyocytes. Cell tracking via fluorescent nanocrystals confirmed the presence of both cell populations in the 50/50 mixed tissues at the time of mechanical characterization.
[Archives of Biochemistry and Biophysics]
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In Silico and In Vitro Studies on the Anti-Cancer Activity of Andrographolide Targeting Survivin in Human Breast Cancer Stem Cells

Human CD24/CD44+ breast cancer stem cells (BCSCs) were treated with andrographolide in vitro for 24 hours. The cytotoxic effect of andrographolide on BCSCs was compared to that on human mesenchymal stem cells.
[PLoS One]
Wanandi, S. I., Limanto, A., Yunita, E., Syahrani, R. A., Louisa, M., Wibowo, A. E., & Arumsari, S. (2020). In silico and in vitro studies on the anti-cancer activity of andrographolide targeting survivin in human breast cancer stem cells. PLOS ONE, 15(11), e0240020. https://doi.org/10.1371/journal.pone.0240020 Cite
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Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19

Researchers analyzed five public scRNA-seq pancreas datasets and performed fluorescence in situ hybridization, Western blotting and immunolocalization for ACE2 with extensive reagent validation on normal human pancreatic tissues across the lifespan, as well as those from COVID-19 cases.
[Cell Metabolism]
Kusmartseva, I., Wu, W., Syed, F., Heide, V. V. D., Jorgensen, M., Joseph, P., Tang, X., Candelario-Jalil, E., Yang, C., Nick, H., Harbert, J. L., Posgai, A. L., Paulsen, J. D., Lloyd, R., Cechin, S., Pugliese, A., Campbell-Thompson, M., Heide, R. S. V., Evans-Molina, C., … Atkinson, M. A. (2020). Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19. Cell Metabolism, 0(0). https://doi.org/10.1016/j.cmet.2020.11.005 Cite
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Targeting PSMD14 Inhibits Melanoma Growth through SMAD3 Stabilization

The authors focused on deubiquitinating enzymes, which regulate protein stability through ubiquitin–proteasome systems, and identified 26S proteasome non-ATPase regulatory subunit 14 (PSMD14) as a molecule related to melanoma growth using siRNA library screening.
[Scientific Reports]
Yokoyama, S., Iwakami, Y., Hang, Z., Kin, R., Zhou, Y., Yasuta, Y., Takahashi, A., Hayakawa, Y., & Sakurai, H. (2020). Targeting PSMD14 inhibits melanoma growth through SMAD3 stabilization. Scientific Reports, 10(1), 19214. https://doi.org/10.1038/s41598-020-76373-y Cite
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MicroRNA-200b/C-3p Regulate Epithelial Plasticity and Inhibit Cutaneous Wound Healing by Modulating TGF-β-Mediated RAC1 Signaling

Investigators focused on microRNA-200b/c-3p whose expression was abundant in intact epidermis, but dramatically decreased in skin wounds.
[Cell Death & Disease]
Tang, H., Wang, X., Zhang, M., Yan, Y., Huang, S., Ji, J., Xu, J., Zhang, Y., Cai, Y., Yang, B., Lan, W., Huang, M., & Zhang, L. (2020). MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling. Cell Death & Disease, 11(10), 1–17. https://doi.org/10.1038/s41419-020-03132-2 Cite
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