induced pluripotent stem cells

[Cell Regeneration] The expression of genes MYF5, MRF4, MYOD is activated by muscle progenitors throughout development, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases of muscle growth.

[NPJ Regenerative Medicine] Scientists reported the establishment of a human congenital cataract in vitro model through differentiation of patient-specific iPSCs into regenerated lenses.

[Science] The past decade has seen considerable advances in in vitro gametogenesis, which aims to recreate germ cell development from PSCs in culture. Mouse PSCs can be induced into functional oocytes and spermatozoa, whereas human PSCs can be induced into early oocytes and prospermatogonia, promoting mechanistic understanding of mammalian germ cell development.

[Cryobiology] Investigators presented a novel vitrification-based method for adherent human iPSCs, designed for automated handling by microfluidic approaches and with ready-to-use potential, in suspension-based bioreactors after thawing, for example.

[Stem Cell Research] Scientists generated iPSCs from a normal individual by electroporation of peripheral blood mononuclear cells, and further generated a SCN1B-knockout human iPSC line via CRISPR/Cas9 gene editing.

[Blood Advances] The authors developed reproducible methods to derive, from human pluripotent stem cells, a population containing monocyte progenitors able to generate functional osteoclast cells. Within this population, they identified cells with monocyte and osteoclast progenitor activity based on CD11b and CD14 expression.