Scientists developed an impedance system to measure the change in electrical resistance of 3D HIOs depending on the integrity of the intestinal epithelial cell membrane, which can reflect functionality and maturity.
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Ahn, J., Jung, K. B., Kwon, O., Choi, M.-S., Ahn, J.-H., Han, H.-Y., Jung, C.-R., Yoon, S., Son, M.-Y., & Oh, J.-H. (2021). Impedance Measurement System for Assessing the Barrier Integrity of Three-Dimensional Human Intestinal Organoids. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.1c00655 Cite
Neutrophil extracellular traps treatment induced intestinal epithelial monolayer barrier disruption and endoplasmic reticulum (ER) stress activation in a dose-dependent manner in vitro, and ER stress inhibition markedly attenuated intestinal apoptosis and tight junction injury.
[Cell Death & Disease]
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Investigators showed that the intestine-enriched miR-802 was a central regulator of intestinal epithelial cell proliferation, Paneth cell function, and enterocyte differentiation.
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Scientists linked intestinal dysbiosis, systemic inflammation, and inflammasome activity to intestinal epithelial cells major histocompatibility complex class II upregulation via an intestinal serum Interleukin-18-interferon gamma axis.
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Scientists examined the function of the RNF20/RNF40/H2Bub1 axis in intestinal inflammation in inflammatory bowel disease (IBD) patients and mouse models. They analyzed underlying molecular pathways in isolated primary intestinal epithelial cells and confirmed their findings in IBD resection specimens.
[Cell Death & Differentiation]
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Researchers developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable human intestinal epithelial cells (hIECs) progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4.
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Kwon, O., Jung, K. B., Lee, K.-R., Son, Y. S., Lee, H., Kim, J.-J., Kim, K., Lee, S., Song, Y.-K., Jung, J., Park, K., Kim, D.-S., Son, M. J., Lee, M.-O., Han, T.-S., Cho, H.-S., Oh, S. J., Chung, H., Kim, S.-H., … Son, M.-Y. (2021). The development of a functional human small intestinal epithelium model for drug absorption. Science Advances, 7(23), eabh1586. https://doi.org/10.1126/sciadv.abh1586 Cite
Researchers analyzed colonic tissue samples from patients with ulcerative colitis (UC) and constructed specific intestinal epithelial phenazine biosynthesis-like domain-containing protein (PBLD)-deficient mice to investigate the role of this protein in UC pathogenesis.
[Cell Death & Disease]
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Scientists examined the relationship of RAB11A to epithelial junctional complexes, YAP, and the associated consequences on colonic epithelial tissue repair.
[Journal of Biological Chemistry]
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Cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) deficiency led to intestinal stem cell loss and compromised intestinal barrier integrity upon dextran sodium sulfate-induced acute injury.
[Proceedings of the National Academy of Sciences of the United States of America]
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Scientists developed a live cell ratiometric fluorescent Optical Stem Cell Activity Reporter (OSCAR). Using the small intestinal crypt as a model, OSCAR revealed in real time the dynamics of dormancy induction and cellular differentiation in vitro, and allowed for the identification and isolation of several populations of transcriptionally diverse OSCARhigh and OSCARlow intestinal epithelial cell states in vivo.
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Investigators generated CYP3A4-expressing Caco-2 cells and attempted to establish a model that could simultaneously evaluate drug absorption and metabolism.
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