Impedance Measurement System for Assessing the Barrier Integrity of Three-Dimensional Human Intestinal Organoids

Scientists developed an impedance system to measure the change in electrical resistance of 3D HIOs depending on the integrity of the intestinal epithelial cell membrane, which can reflect functionality and maturity.
[Analytical Chemistry]
Ahn, J., Jung, K. B., Kwon, O., Choi, M.-S., Ahn, J.-H., Han, H.-Y., Jung, C.-R., Yoon, S., Son, M.-Y., & Oh, J.-H. (2021). Impedance Measurement System for Assessing the Barrier Integrity of Three-Dimensional Human Intestinal Organoids. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.1c00655 Cite
AbstractGraphical Abstract
Bookmark

No account yet? Register

0
Share

Neutrophil Extracellular Traps Impair Intestinal Barrier Functions in Sepsis by Regulating TLR9-Mediated Endoplasmic Reticulum Stress Pathway

Neutrophil extracellular traps treatment induced intestinal epithelial monolayer barrier disruption and endoplasmic reticulum (ER) stress activation in a dose-dependent manner in vitro, and ER stress inhibition markedly attenuated intestinal apoptosis and tight junction injury.
[Cell Death & Disease]
Full Article
Bookmark

No account yet? Register

0
Share

miR-802 Regulates Paneth Cell Function and Enterocyte Differentiation in the Mouse Small Intestine

Investigators showed that the intestine-enriched miR-802 was a central regulator of intestinal epithelial cell proliferation, Paneth cell function, and enterocyte differentiation.
[Nature Communications]
Full Article
Bookmark

No account yet? Register

0
Share

Genetic and Commensal Induction of IL-18 Drive Intestinal Epithelial MHCII via IFNγ

Scientists linked intestinal dysbiosis, systemic inflammation, and inflammasome activity to intestinal epithelial cells major histocompatibility complex class II upregulation via an intestinal serum Interleukin-18-interferon gamma axis.
[Mucosal Immunology]
Abstract
Bookmark

No account yet? Register

0
Share

RNF20 and RNF40 Regulate Vitamin D Receptor-Dependent Signaling in Inflammatory Bowel Disease

Scientists examined the function of the RNF20/RNF40/H2Bub1 axis in intestinal inflammation in inflammatory bowel disease (IBD) patients and mouse models. They analyzed underlying molecular pathways in isolated primary intestinal epithelial cells and confirmed their findings in IBD resection specimens.
[Cell Death & Differentiation]
Full Article
Bookmark

No account yet? Register

0
Share

The Development of a Functional Human Small Intestinal Epithelium Model for Drug Absorption

Researchers developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable human intestinal epithelial cells (hIECs) progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4.
[Science Advances]
Kwon, O., Jung, K. B., Lee, K.-R., Son, Y. S., Lee, H., Kim, J.-J., Kim, K., Lee, S., Song, Y.-K., Jung, J., Park, K., Kim, D.-S., Son, M. J., Lee, M.-O., Han, T.-S., Cho, H.-S., Oh, S. J., Chung, H., Kim, S.-H., … Son, M.-Y. (2021). The development of a functional human small intestinal epithelium model for drug absorption. Science Advances, 7(23), eabh1586. https://doi.org/10.1126/sciadv.abh1586 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Epithelial PBLD Attenuates Intestinal Inflammatory Response and Improves Intestinal Barrier Function by Inhibiting NF-κB Signaling

Researchers analyzed colonic tissue samples from patients with ulcerative colitis (UC) and constructed specific intestinal epithelial phenazine biosynthesis-like domain-containing protein (PBLD)-deficient mice to investigate the role of this protein in UC pathogenesis.
[Cell Death & Disease]
Full Article
Bookmark

No account yet? Register

0
Share

RAB11A-Mediated YAP Localization to Adherens and Tight Junctions Is Essential for Colonic Epithelial Integrity

Scientists examined the relationship of RAB11A to epithelial junctional complexes, YAP, and the associated consequences on colonic epithelial tissue repair.
[Journal of Biological Chemistry]
Full Article
Bookmark

No account yet? Register

0
Share

cGAS Restricts Colon Cancer Development by Protecting Intestinal Barrier Integrity

Cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) deficiency led to intestinal stem cell loss and compromised intestinal barrier integrity upon dextran sodium sulfate-induced acute injury.
[Proceedings of the National Academy of Sciences of the United States of America]
Full Article
Bookmark

No account yet? Register

0
Share

Establishment of a Fluorescent Reporter of RNA-Polymerase II Activity to Identify Dormant Cells

Scientists developed a live cell ratiometric fluorescent Optical Stem Cell Activity Reporter (OSCAR). Using the small intestinal crypt as a model, OSCAR revealed in real time the dynamics of dormancy induction and cellular differentiation in vitro, and allowed for the identification and isolation of several populations of transcriptionally diverse OSCARhigh and OSCARlow intestinal epithelial cell states in vivo.
[Nature Communications]
Full Article
Bookmark

No account yet? Register

0
Share

Generation of Tetracycline-Controllable CYP3A4-Expressing Caco-2 Cells by the piggyBac Transposon System

Investigators generated CYP3A4-expressing Caco-2 cells and attempted to establish a model that could simultaneously evaluate drug absorption and metabolism.
[Scientific Reports]
Full Article
Bookmark

No account yet? Register

0
Share
Share