Researchers investigated mechanisms by which PAF1 maintains cancer stem cells and contributes to development of pancreatic tumors.
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Karmakar, S., Rauth, S., Nallasamy, P., Perumal, N., Nimmakalaya, R. K., Leon, F., Gupta, R., Barkeer, S., Venkata, R. C., Raman, V., Rachagani, S., Ponnusamy, M. P., & Batra, S. K. (2020). PAF1 Regulates Stem Cell Features of Pancreatic Cancer Cells, Independently of the PAF1 Complex, via Interactions with PHF5A and DDX3. Gastroenterology, 0(0). https://doi.org/10.1053/j.gastro.2020.07.053 Cite
Scientists used metabolic labeling with stable isotopes and mass spectrometry to quantitatively address the global turnover of genomic 5-methyl-2′-deoxycytidine, 5-hydroxymethyl-2′-deoxycytidine and 5-formyl-2′-deoxycytidine across mouse pluripotent cell states.
[Nature Chemical Biology]
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Investigators tested three exosomes isolation methods and detergents for solubilization of exosomal proteins and enhanced detection by mass spectrometry.
M1 and M2 macrophage viability and activation were mainly found to be unaffected by few‐layer graphene and MoS2 at doses up to 50 µg mL−1.
Researchers showed that sex-determining region Y-box 9 (SOX9) was up-regulated after adenosine 5′-triphosphate treatment in breast cancer cells. In vitro invasion and migration assays demonstrated that knocking down SOX9 attenuated ATP-driven invasive capability.
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Scientists found that incorporation of the CD3ε cytoplasmic domain into a second-generation chimeric antigen receptor (CAR) improved antitumor activity of CAR-T cells.
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Wu, W., Zhou, Q., Masubuchi, T., Shi, X., Li, H., Xu, X., Huang, M., Meng, L., He, X., Zhu, H., Gao, S., Zhang, N., Jing, R., Sun, J., Wang, H., Hui, E., Wong, C. C., & Xu, C. (2020). Multiple Signaling Roles of CD3ε and Its Application in CAR-T Cell Therapy. Cell, 0(0). https://doi.org/10.1016/j.cell.2020.07.018 Cite
By taking advantage of a human induced pluripotent stem cell-based model, scientists aimed to gain insights on the impact of amyotrophic lateral sclerosis (ALS) mutant FUS on the motoneuron proteome.
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