Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons

The authors identified and isolated human iPSC-dopamine neurons with a TH-RFP reporter. Using liquid extraction surface analysis (LESA) coupled with high-resolution mass spectrometry they developed a high-throughput method for untargeted single cell lipid profiling.
[iScience]
Snowden, S. G., Fernandes, H. J. R., Kent, J., Foskolou, S., Tate, P., Field, S. F., Metzakopian, E., & Koulman, A. (2020). Development and application of high-throughput single cell lipid profiling: a study of SNCA-A53T human dopamine neurons. IScience, 0(0). https://doi.org/10.1016/j.isci.2020.101703 Cite
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Identification of Drivers of Breast Cancer Invasion by Secretome Analysis: Insight Into CTGF Signaling

Scientists combined data from secretome and proteome analysis using mass spectrometry with microarray data from mesenchymal transformed breast cancer cells to elucidate the drivers of epithelial-mesenchymal transition and cell invasion.
[Scientific Reports]
Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling | Scientific Reports. (n.d.). Retrieved October 21, 2020, from https://www.nature.com/articles/s41598-020-74838-8 Cite
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EVI1 Phosphorylation at S436 Regulates Interactions with CtBP1 and DNMT3A and Promotes Self-Renewal

Wild-type EVI1 with S436 available for phosphorylation, but not non-phosphorylatable EVI1-S436A, conferred hematopoietic progenitor cell self-renewal and was associated with significantly higher organized transcriptional patterns.
[Cell Death & Disease]
Paredes, R., Kelly, J. R., Geary, B., Almarzouq, B., Schneider, M., Pearson, S., Narayanan, P., Williamson, A., Lovell, S. C., Wiseman, D. H., Chadwick, J. A., Jones, N. J., Kustikova, O., Schambach, A., Garner, T., Amaral, F. M. R., Pierce, A., Stevens, A., Somervaille, T. C. P., … Meyer, S. (2020). EVI1 phosphorylation at S436 regulates interactions with CtBP1 and DNMT3A and promotes self-renewal. Cell Death & Disease, 11(10), 1–14. https://doi.org/10.1038/s41419-020-03099-0 Cite
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CHIP-Mediated CIB1 Ubiquitination Regulated Epithelial–Mesenchymal Transition and Tumor Metastasis in Lung Adenocarcinoma

The expression levels of CIB1 in lung adenocarcinoma tissues and adjacent normal tissues were examined by immunohistochemistry, and the relationship between CIB1 expression and patient clinicopathological characteristics was analyzed.
[Cell Death & Differentiation]
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CASZ1b Is a Novel Transcriptional Corepressor of Mineralocorticoid Receptor

Knockdown of CASZ1b via RNA interference increased the expression levels of the aldosterone-induced MR target genes epithelial Na+ channel-α and serum/glucocorticoid regulated kinase 1 by approximately twofold and 2.3-fold.
[Hypertension Research]
Yokota, K., Shibata, H., Kurihara, I., Kobayashi, S., Murai-Takeda, A., & Itoh, H. (2020). CASZ1b is a novel transcriptional corepressor of mineralocorticoid receptor. Hypertension Research, 1–10. https://doi.org/10.1038/s41440-020-00562-5 Cite
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Cell-Free DNA in Plasma as an Essential Immune System Regulator

Researchers showed that cell-free DNA presence and clearance in plasma of healthy individuals played an indispensable role in immune system regulation.
[Scientific Reports]
Korabecna, M., Zinkova, A., Brynychova, I., Chylikova, B., Prikryl, P., Sedova, L., Neuzil, P., & Seda, O. (2020). Cell-free DNA in plasma as an essential immune system regulator. Scientific Reports, 10(1), 17478. https://doi.org/10.1038/s41598-020-74288-2 Cite
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Cystatin C Regulates the Cytotoxicity of Infection‐Induced Endothelial‐Derived β‐Amyloid

Mass spectrometry of endothelial cell culture media following P. aeruginosa infection allowed identification of multiple potential secreted modulators of beta amyloid, including cystatin C, gelsolin, and ApoJ/clusterin.
[Febs Open Bio]
Balczon, R., Morrow, K. A., Leavesley, S., Francis, C. M., Stevens, T. C., Agwaramgbo, E., Williams, C., Stevens, R. P., Langham, G., Voth, S., Cioffi, E. A., Weintraub, S. E., & Stevens, T. (n.d.). Cystatin C regulates the cytotoxicity of infection-induced endothelial-derived β-amyloid. FEBS Open Bio, n/a(n/a). https://doi.org/10.1002/2211-5463.12997 Cite
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Crystallographic and Electrophilic Fragment Screening of the SARS-CoV-2 Main Protease

Researchers performed a large-scale screen of electrophile and non-covalent fragments through a combined mass spectrometry and X-ray approach against the SARS-CoV-2 main protease, one of two cysteine viral proteases essential for viral replication.
[Nature Communications]
Douangamath, A., Fearon, D., Gehrtz, P., Krojer, T., Lukacik, P., Owen, C. D., Resnick, E., Strain-Damerell, C., Aimon, A., Ábrányi-Balogh, P., Brandão-Neto, J., Carbery, A., Davison, G., Dias, A., Downes, T. D., Dunnett, L., Fairhead, M., Firth, J. D., Jones, S. P., … Walsh, M. A. (2020). Crystallographic and electrophilic fragment screening of the SARS-CoV-2 main protease. Nature Communications, 11(1), 5047. https://doi.org/10.1038/s41467-020-18709-w Cite
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Staphylococcus aureus Second Immunoglobulin-Binding Protein Drives Atopic Dermatitis via IL-33

In vitro human keratinocyte cell culture, ex vivo human skin organ explants and the eczema prone Nc/Tnd mouse were used as model systems to assess type-2 promoting immune responses to S. aureus.
[Journal of Allergy and Clinical Immunology]
Kindi, A. A., Williams, H., Matsuda, K., Alkahtani, A. M., Saville, C., Bennett, H., Alshammari, Y., Tan, S. Y., O’Neill, C., Tanaka, A., Matsuda, H., Arkwright, P. D., & Pennock, J. L. (2020). Staphylococcus aureus Second Immunoglobulin-Binding Protein drives atopic dermatitis via IL-33. Journal of Allergy and Clinical Immunology, 0(0). https://doi.org/10.1016/j.jaci.2020.09.023 Cite
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A Functional Genomic Screen Identifies the Deubiquitinase USP11 as a Novel Transcriptional Regulator of ERα in Breast Cancer

Scientists investigated the role of deubiquitinases (DUB) in regulating estrogen receptor α (ERα) in breast cancer. An RNAi loss-of-function screen in breast cancer cells targeting all DUB identified USP11 was a regulator of ERα transcriptional activity, which was further validated by assessment of direct transcriptional targets of ERα.
[Cancer Research]
Dwane, L., O’Connor, A. E., Das, S., Moran, B., Mulrane, L., Pinto-Fernandez, A., Ward, E., Blümel, A. M., Cavanagh, B. L., Mooney, B., Dirac, A. M., Jirström, K., Kessler, B. M., Chonghaile, T. N., Bernards, R., Gallagher, W. M., & O’Connor, D. P. (2020). A functional genomic screen identifies the deubiquitinase USP11 as a novel transcriptional regulator of ERα in breast cancer. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-20-0214 Cite
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A Defined N6-Methyladenosine (m6A) Profile Conferred by METTL3 Regulates Muscle Stem Cell/Myoblast State Transitions

RNAi studies showed that reducing levels of METTL3, the active m6A methyltransferase, reduced global m6A levels and forced C2C12 myoblasts to prematurely differentiate.
[Cell Death Discovery]
Gheller, B. J., Blum, J. E., Fong, E. H. H., Malysheva, O. V., Cosgrove, B. D., & Thalacker-Mercer, A. E. (2020). A defined N6-methyladenosine (m 6 A) profile conferred by METTL3 regulates muscle stem cell/myoblast state transitions. Cell Death Discovery, 6(1), 1–14. https://doi.org/10.1038/s41420-020-00328-5 Cite
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