Real-Time Lipid Patterns to Classify Viable and Necrotic Liver Tumors

The authors explored whether rapid evaporative ionization mass spectrometry lipid patterns ccould identify human liver tumors and improve the rapid characterization of their underlying metabolic features.
[Laboratory Investigation]
Vaysse, P.-M., Grabsch, H. I., van den Hout, M. F. C. M., Bemelmans, M. H. A., Heeren, R. M. A., Olde Damink, S. W. M., & Porta Siegel, T. (2021). Real-time lipid patterns to classify viable and necrotic liver tumors. Laboratory Investigation, 1–15. https://doi.org/10.1038/s41374-020-00526-w Cite
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Substrate-Specific Recognition of IKKs Mediated by USP16 Facilitates Autoimmune Inflammation

Investigators found that IKKβ ubiquitination on lysine-238 was substantially increased during inflammation. Using mass spectrometry, they identified USP16 as an essential regulator of the IKKβ ubiquitination level that selectively affected p105 phosphorylation without directly affecting p65 or IκBα phosphorylation.
[Science Advances]
Substrate-specific recognition of IKKs mediated by USP16 facilitates autoimmune inflammation | Science Advances. (n.d.). Retrieved January 22, 2021, from https://advances.sciencemag.org/content/7/3/eabc4009 Cite
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EEF1A2 Interacts with HSP90AB1 to Promote Lung Adenocarcinoma Metastasis via Enhancing TGF-β/SMAD Signaling

Scientists report that eukaryotic protein translation elongation factor 1α2 (EEF1A2) mediates the epithelial–mesenchymal transformation, to promote the metastasis of lung adenocarcinoma cells in vitro and in vivo.
[British Journal of Cancer]
Jia, L., Ge, X., Du, C., Chen, L., Zhou, Y., Xiong, W., Xiang, J., Li, G., Xiao, G., Fang, L., & Li, Z. (2021). EEF1A2 interacts with HSP90AB1 to promote lung adenocarcinoma metastasis via enhancing TGF-β/SMAD signalling. British Journal of Cancer, 1–11. https://doi.org/10.1038/s41416-020-01250-4 Cite
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Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton’s Jelly

The authors characterized the protein secretome of adipose, bone marrow, placenta, and Wharton’s jelly-derived human MSCs by using conditioned media and analyzing the secretome by mass spectrometry and follow-up bioinformatics.
[International Journal of Molecular Sciences]
Shin, S., Lee, J., Kwon, Y., Park, K.-S., Jeong, J.-H., Choi, S.-J., Bang, S. I., Chang, J. W., & Lee, C. (2021). Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton’s Jelly. International Journal of Molecular Sciences, 22(2), 845. https://doi.org/10.3390/ijms22020845 Cite
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SUMOylation Disassembles the Tetrameric Pyruvate Kinase M2 to Block Myeloid Differentiation of Leukemia Cells

Scientists found that SUMOylation of the M2 isoform of pyruvate kinase, a rate-limiting glycolytic enzyme catalyzing the dephosphorylation of phosphoenolpyruvate to pyruvate, is prevalent in a variety of leukemic cell lines as well as primary samples from patients with leukemia through multiple-reaction monitoring based targeted mass spectrometry analysis.
[Cell Death & Disease]
Xia, L., Jiang, Y., Zhang, X.-H., Wang, X.-R., Wei, R., Qin, K., & Lu, Y. (2021). SUMOylation disassembles the tetrameric pyruvate kinase M2 to block myeloid differentiation of leukemia cells. Cell Death & Disease, 12(1), 1–13. https://doi.org/10.1038/s41419-021-03400-9 Cite
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Proteomics Analysis of Human Intestinal Organoids during Hypoxia and Reoxygenation as a Model to Study Ischemia-Reperfusion Injury

Investigators demonstrated the use of human small intestinal organoids to model ischemia-reperfusion injury by exposing organoids to hypoxia and reoxygenation.
[Cell Death & Disease]
Kip, A. M., Soons, Z., Mohren, R., Duivenvoorden, A. A. M., Röth, A. A. J., Cillero-Pastor, B., Neumann, U. P., Dejong, C. H. C., Heeren, R. M. A., Olde Damink, S. W. M., & Lenaerts, K. (2021). Proteomics analysis of human intestinal organoids during hypoxia and reoxygenation as a model to study ischemia-reperfusion injury. Cell Death & Disease, 12(1), 1–13. https://doi.org/10.1038/s41419-020-03379-9 Cite
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Multiple Screening Approaches Reveal HDAC6 as a Novel Regulator of Glycolytic Metabolism in Triple-Negative Breast Cancer

Investigators set up a phenotypic small-molecule screen to reveal vulnerabilities in TNBC cells that were independent of mitochondrial apoptosis. Using a functional genetic approach, they identified that a “hit” compound, BAS-2, had a potentially similar mechanism of action to histone deacetylase inhibitors.
[Science Advances]
Dowling, C. M., Hollinshead, K. E. R., Grande, A. D., Pritchard, J., Zhang, H., Dillon, E. T., Haley, K., Papadopoulos, E., Mehta, A. K., Bleach, R., Lindner, A. U., Mooney, B., Düssmann, H., O’Connor, D., Prehn, J. H. M., Wynne, K., Hemann, M., Bradner, J. E., Kimmelman, A. C., … Chonghaile, T. N. (2021). Multiple screening approaches reveal HDAC6 as a novel regulator of glycolytic metabolism in triple-negative breast cancer. Science Advances, 7(3), eabc4897. https://doi.org/10.1126/sciadv.abc4897 Cite
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Lnc-Rps4l-Encoded Peptide RPS4XL Regulates RPS6 Phosphorylation Inhibits the Proliferation of Pulmonary Artery Smooth Muscle Cells Caused by Hypoxia

Researchers hypothesized in this study that an lnc-Rps4l-encoded peptide is involved in hypoxic induced pulmonary artery smooth muscle cells (PASMCs) proliferation. The presence of peptide RPS4XL encoded by lnc-Rps4l in PASMCs under hypoxic conditions were confirmed by bioinformatics, immunofluorescence and immunohistochemistry.
[Molecular Therapy]
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ACPA Decreases Non-Small Cell Lung Cancer Line Growth through Akt/PI3K and JNK Pathways In Vitro

Investigators confirmed that CB1 receptor (CB1R) was expressed in non-small cell lung cancer cells in this study. Arachidonoylcyclopropylamide (ACPA) as a synthetic, CB1R-specific ligand decreased proliferation rate in NSCLC cells by WST-1 analysis and real-time proliferation assay.
[Cell Death & Disease]
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Octenidine-Based Hydrogel Shows Anti-Inflammatory and Protease-Inhibitory Capacities in Wounded Human Skin

Proteomics analysis of octenidine dihydrochloride-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines and matrix-metalloproteinases when compared to controls.
[Scientific Reports]
Seiser, S., Janker, L., Zila, N., Mildner, M., Rakita, A., Matiasek, J., Bileck, A., Gerner, C., Paulitschke, V., & Elbe-Bürger, A. (2021). Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin. Scientific Reports, 11(1), 32. https://doi.org/10.1038/s41598-020-79378-9 Cite
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Proteomic Analysis and Cell Viability of Nine Amnion, Chorion, Umbilical Cord, and Amniotic Fluid–Derived Products

Investigators determined the full proteome and examine cell viability in nine commercial amnion products using an unbiased bottom-up shotgun proteomics approach and confocal microscopy.
[Cartilage]
Becktell, L., Matuska, A. M., Hon, S., Delco, M. L., Cole, B. J., Begum, L., Zhang, S., & Fortier, L. A. (2020). Proteomic Analysis and Cell Viability of Nine Amnion, Chorion, Umbilical Cord, and Amniotic Fluid–Derived Products. CARTILAGE, 1947603520976767. https://doi.org/10.1177/1947603520976767 Cite
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