Extensive Remodeling of the Extracellular Matrix during Aging Contributes to Age-Dependent Impairments of Muscle Stem Cell Functionality

Scientists used quantitative mass spectrometry to characterize intrinsic changes in the muscle stem cell (MuSC) proteome and remodeling of the MuSC niche during aging.
[Cell Reports]
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Apoptotic Mesenchymal Stromal Cells Support Osteoclastogenesis While Inhibiting Multinucleated Giant Cells Formation In Vitro

Scientists focused on the communication from MSCs towards osteoclasts-like cells in vitro. MSCs seeded on a calcium-phosphate biomaterial or undergoing induced apoptosis produced a conditioned media favoring the development of osteoclasts from human CD14+ monocytes.
[Scientific Reports]
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Valosin-Containing Protein Stabilizes Mutant p53 to Promote Pancreatic Cancer Growth

The authors identified valosin-containing protein as a regulator of p53-R273H by conducting immunoprecipitation-tandem mass spectrometry analysis. VCP bound p53-R273H at its DNA binding domain.
[Cancer Research]
Wang, J., Chen, Y., Huang, C., Hao, Q., Zeng, S. X., Omari, S., Zhang, Y., Zhou, X., & Lu, H. (2021). Valosin-containing protein stabilizes mutant p53 to promote pancreatic cancer growth. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-20-3855 Cite
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MMP8 Increases Tongue Carcinoma Cell–Cell Adhesion and Diminishes Migration via Cleavage of Anti-Adhesive FXYD5

The authors mapped the substrates of matrix metalloproteinase 8 (MMP8) to elucidate its previously shown tumor-protective role in oral tongue squamous cell carcinoma. MMP8 overexpressing (+) HSC-3 cells, previously demonstrated to have reduced migration and invasion, showed enhanced cell-cell adhesion.
[Oncogenesis]
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LncRNA H19 Governs Mitophagy and Restores Mitochondrial Respiration in the Heart through Pink1/Parkin Signaling during Obesity

The effects of long non-coding RNA H19 (H19) on metabolic disorders, mitochondrial respiratory function, and mitophagy were investigated. Researchers suggested that DNA methylation-mediated downregulation of H19 expression played a crucial role in cardiomyocyte metabolic disorders.
[Cell Death & Disease]
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Continual Proteomic Divergence of HepG2 Cells as a Consequence of Long-Term Spheroid Culture

Researchers assessed the influence of culture duration on the relative proteome abundance of HepG2 cells cultured as spheroids, which are routinely used to model aspects of the liver.
[Scientific Reports]
Ellero, A. A., van den Bout, I., Vlok, M., Cromarty, A. D., & Hurrell, T. (2021). Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture. Scientific Reports, 11(1), 10917. https://doi.org/10.1038/s41598-021-89907-9 Cite
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Oncogenic KRAS Engages an RSK1/NF1 Pathway to Inhibit Wild-Type RAS Signaling in Pancreatic Cancer

Investigators used proximity labeling to identify protein interactors of active KRAS in pancreatic ductal adenocarcinoma (PDAC) cells. We expressed fusions of wild-type (WT) (BirA-KRAS4B), mutant (BirA-KRAS4BG12D), and nontransforming cytosolic double mutant (BirA-KRAS4BG12D/C185S) KRAS with the BirA biotin ligase in murine PDAC cells.
[Proceedings of the National Academy of Sciences of the United States of America]
Cheng, D. K., Oni, T. E., Thalappillil, J. S., Park, Y., Ting, H.-C., Alagesan, B., Prasad, N. V., Addison, K., Rivera, K. D., Pappin, D. J., Aelst, L. V., & Tuveson, D. A. (2021). Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer. Proceedings of the National Academy of Sciences, 118(21). https://doi.org/10.1073/pnas.2016904118 Cite
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The Arachidonic Acid Metabolite 11,12-Epoxyeicosatrienoic Acid Alleviates Pulmonary Fibrosis

Liquid chromatography with tandem mass spectrometry was used to analyze epoxyeicosatrienoic acids (EETs) in the lung tissues of patients with idiopathic pulmonary fibrosis and controls, and the function of 11,12-EET was evaluated in in vitro and in vivo in pulmonary fibrosis models.
[Environmental & Molecular Medicine]
Kim, H. S., Moon, S.-J., Lee, S. E., Hwang, G. W., Yoo, H. J., & Song, J. W. (2021). The arachidonic acid metabolite 11,12-epoxyeicosatrienoic acid alleviates pulmonary fibrosis. Experimental & Molecular Medicine, 1–11. https://doi.org/10.1038/s12276-021-00618-7 Cite
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Single-Cell Lipidomics with High Structural Specificity by Mass Spectrometry

Scientists developed a strategy that enabled single-cell lipidomic analysis with high structural specificity and classified four subtypes of human breast cancer cells.
[Nature Communications]
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Phytochemicals from Ajwa Dates Pulp Extract Induce Apoptosis in Human Triple-Negative Breast Cancer by Inhibiting AKT/mTOR Pathway and Modulating Bcl-2 Family Proteins

Researchers analyzed the phytoconstituents in ethanolic Ajwa Dates Pulp Extract by liquid chromatography-mass spectrometry and investigated anticancer effects against MDA-MB-231 cells.
[Scientific Reports]
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Cardiomyocyte Contractile Impairment in Heart Failure Results from Reduced BAG3-Mediated Sarcomeric Protein Turnover

Using mass spectrometry, the authors characterized the myofilament chaperone-assisted selective autophagy interactome in the human heart and identified eight clients of BAG3-mediated protein turnover.
[Nature Communications]
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