Dissection of Two Routes to Naïve Pluripotency Using Different Kinase Inhibitors

Researchers use mass spectrometry to describe the molecular events triggered by inhibition of Mek1/2 and Gsk3 and inhibition of Cdk8/19 on ESCs.
[Nature Communications]
Martinez-Val, A., Lynch, C. J., Calvo, I., Ximénez-Embún, P., Garcia, F., Zarzuela, E., Serrano, M., & Munoz, J. (2021). Dissection of two routes to naïve pluripotency using different kinase inhibitors. Nature Communications, 12(1), 1863. https://doi.org/10.1038/s41467-021-22181-5 Cite
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Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies

Investigators developed two hiPSC/hESC-specific glycan-recognizing mouse antibodies, R-10G and R-17F, using the Tic hiPSC line as an antigen. R-10G recognized a low-sulfate keratan sulfate, and R-17F recognized lacto-N-fucopentaose-1.
[Biomolecules]
Nagai, Y., Nakao, H., Kojima, A., Komatsubara, Y., Ohta, Y., Kawasaki, N., Kawasaki, N., Toyoda, H., & Kawasaki, T. (2021). Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies. Biomolecules, 11(4), 508. https://doi.org/10.3390/biom11040508 Cite
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Structural Basis of Malaria RIFIN Binding by LILRB1-Containing Antibodies

By screening plasma from a cohort of donors from Mali, researchers identified individuals with LILRB1-containing antibodies.
[Nature]
Chen, Y., Xu, K., Piccoli, L., Foglierini, M., Tan, J., Jin, W., Gorman, J., Tsybovsky, Y., Zhang, B., Traore, B., Silacci-Fregni, C., Daubenberger, C., Crompton, P. D., Geiger, R., Sallusto, F., Kwong, P. D., & Lanzavecchia, A. (2021). Structural basis of malaria RIFIN binding by LILRB1-containing antibodies. Nature, 1–5. https://doi.org/10.1038/s41586-021-03378-6 Cite
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Ontogenic Shifts in Cellular Fate Are Linked to Proteotype Changes in Lineage-Biased Hematopoietic Progenitor Cells

Through deep coverage of the cellular proteome of fetal and adult lympho-myeloid multipotent progenitors, common lymphoid progenitors, and granulocyte-monocyte progenitors, researchers established that features traditionally attributed to adult hematopoiesis were conserved across lymphoid and myeloid lineages.
[Cell Reports]
Jassinskaja, M., Pimková, K., Arh, N., Johansson, E., Davoudi, M., Pereira, C.-F., Sitnicka, E., & Hansson, J. (2021). Ontogenic shifts in cellular fate are linked to proteotype changes in lineage-biased hematopoietic progenitor cells. Cell Reports, 34(12). https://doi.org/10.1016/j.celrep.2021.108894 Cite
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Global Proteomic Analysis of Extracellular Matrix in Mouse and Human Brain Highlights Relevance to Cerebrovascular Disease

Scientists developed an approach to extract the cerebrovascular extracellular matrix from mouse and human post-mortem normal brain tissues.
[Journal of Cerebral Blood Flow and Metabolism]
Pokhilko, A., Brezzo, G., Handunnetthi, L., Heilig, R., Lennon, R., Smith, C., Allan, S. M., Granata, A., Sinha, S., Wang, T., Markus, H. S., Naba, A., Fischer, R., Van Agtmael, T., Horsburgh, K., & Cader, M. Z. (2021). Global proteomic analysis of extracellular matrix in mouse and human brain highlights relevance to cerebrovascular disease. Journal of Cerebral Blood Flow & Metabolism, 0271678X211004307. https://doi.org/10.1177/0271678X211004307 Cite
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A Tumor-Suppressive Circular RNA Mediates Uncanonical Integrin Degradation by the Proteasome in Liver Cancer

Investigators showed that polyadenylate-binding protein 1 (circPABPC1) was preferentially lost in tumor cells from clinical samples and inhibited both intrahepatic and distant metastases in a mouse xenograft model.
[Science Advances]
Shi, L., Liu, B., Shen, D., Yan, P., Zhang, Y., Tian, Y., Hou, L., Jiang, G., Zhu, Y., Liang, Y., Liang, X., Shen, B., Yu, H., Zhang, Y., Wang, Y., Guo, X., & Cai, X. (2021). A tumor-suppressive circular RNA mediates uncanonical integrin degradation by the proteasome in liver cancer. Science Advances, 7(13), eabe5043. https://doi.org/10.1126/sciadv.abe5043 Cite
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Long-Term Evolution of the Epithelial Cell Secretome in Preclinical 3D Models of the Human Bronchial Epithelium

Investigators developed a 3D model of the human bronchial epithelium using Calu-3 cell line and demonstrated its viability and functionality for 21 days without subculturing.
[Scientific Reports]
Sanchez-Guzman, D., Boland, S., Brookes, O., Mc Cord, C., Lai Kuen, R., Sirri, V., Baeza Squiban, A., & Devineau, S. (2021). Long-term evolution of the epithelial cell secretome in preclinical 3D models of the human bronchial epithelium. Scientific Reports, 11(1), 6621. https://doi.org/10.1038/s41598-021-86037-0 Cite
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Proteomic/Transcriptomic Analysis of Erythropoiesis

The authors review recent proteomic studies that have furthered our understanding of erythropoiesis with a focus on quantitative mass spectrometry approaches to measure the abundance of transcription factors and cofactors during differentiation.
[Current Opinion in Hematology]
Brand, M., & Ranish, J. A. (2021). Proteomic/transcriptomic analysis of erythropoiesis. Current Opinion in Hematology, Publish Ahead of Print. https://doi.org/10.1097/MOH.0000000000000647 Cite
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SWATH-MS Proteomics of PANC-1 and MIA PaCa-2 Pancreatic Cancer Cells Allows Identification of Drug Targets Alternative to MEK and PI3K Inhibition

Scientists compared PANC-1 and MIA PaCa-2 pancreatic cancer cells which are, respectively, resistant and sensitive to MEK- and PI3K-targeted therapy.
[Biochemical and Biophysical Research Communications]
Aguilar-Valdés, A., Noriega, L. G., Tovar, A. R., Ibarra-Sánchez, M. de J., Sosa-Hernández, V. A., Maravillas-Montero, J. L., & Martínez-Aguilar, J. (2021). SWATH-MS proteomics of PANC-1 and MIA PaCa-2 pancreatic cancer cells allows identification of drug targets alternative to MEK and PI3K inhibition. Biochemical and Biophysical Research Communications, 552, 23–29. https://doi.org/10.1016/j.bbrc.2021.03.018 Cite
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Novel Candidate Factors Predicting the Effect of S-1 Adjuvant Chemotherapy of Pancreatic Cancer

Collection of an adequate number of PDAC cells is difficult due to the surrounding fibroblasts. The authors discovered novel biomarkers to predict chemosensitivity based on the collagen gel droplet-embedded drug sensitivity test results.
[Scientific Reports]
Mitachi, K., Ariake, K., Shima, H., Sato, S., Miura, T., Maeda, S., Ishida, M., Mizuma, M., Ohtsuka, H., Kamei, T., Igarashi, K., & Unno, M. (2021). Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer. Scientific Reports, 11(1), 6541. https://doi.org/10.1038/s41598-021-86099-0 Cite
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Proteomics Investigation of Cbl and Cbl-B in Neuroblastoma Cell Differentiation Highlights Roles for SHP2 and CDK16

The authors studied the role of E3 ubiquitin ligases Cbl and Cbl-b in regulation of long-term signaling responses associated with ERK phosphorylation and neurite outgrowth, a morphological marker of neuroblastoma cell differentiation.
[iScience]
Pedersen, A.-K., Pfeiffer, A., Karemore, G., Akimov, V., Bekker-Jensen, D. B., Blagoev, B., Francavilla, C., & Olsen, J. V. (2021). Proteomics investigation of Cbl and Cbl-b in neuroblastoma cell differentiation highlights roles for SHP2 and CDK16. IScience, 0(0). https://doi.org/10.1016/j.isci.2021.102321 Cite
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