Scientists used quantitative mass spectrometry to characterize intrinsic changes in the muscle stem cell (MuSC) proteome and remodeling of the MuSC niche during aging.
6445212 XQSHYQ42 items 1 apa default asc 1
Scientists focused on the communication from MSCs towards osteoclasts-like cells in vitro. MSCs seeded on a calcium-phosphate biomaterial or undergoing induced apoptosis produced a conditioned media favoring the development of osteoclasts from human CD14+ monocytes.
6445212 PM3DDC3X items 1 apa default asc 1
The authors identified valosin-containing protein as a regulator of p53-R273H by conducting immunoprecipitation-tandem mass spectrometry analysis. VCP bound p53-R273H at its DNA binding domain.
The authors mapped the substrates of matrix metalloproteinase 8 (MMP8) to elucidate its previously shown tumor-protective role in oral tongue squamous cell carcinoma. MMP8 overexpressing (+) HSC-3 cells, previously demonstrated to have reduced migration and invasion, showed enhanced cell-cell adhesion.
6445212 243L5AFB items 1 apa default asc 1
The effects of long non-coding RNA H19 (H19) on metabolic disorders, mitochondrial respiratory function, and mitophagy were investigated. Researchers suggested that DNA methylation-mediated downregulation of H19 expression played a crucial role in cardiomyocyte metabolic disorders.
[Cell Death & Disease]
6445212 QDTWJRWA items 1 apa default asc 1
Researchers assessed the influence of culture duration on the relative proteome abundance of HepG2 cells cultured as spheroids, which are routinely used to model aspects of the liver.
7753456 M4T9F5VV items 1 apa default asc 1
Investigators used proximity labeling to identify protein interactors of active KRAS in pancreatic ductal adenocarcinoma (PDAC) cells. We expressed fusions of wild-type (WT) (BirA-KRAS4B), mutant (BirA-KRAS4BG12D), and nontransforming cytosolic double mutant (BirA-KRAS4BG12D/C185S) KRAS with the BirA biotin ligase in murine PDAC cells.
[Proceedings of the National Academy of Sciences of the United States of America]
7753456 TQHXVBP4 items 1 apa default asc 1
Cheng, D. K., Oni, T. E., Thalappillil, J. S., Park, Y., Ting, H.-C., Alagesan, B., Prasad, N. V., Addison, K., Rivera, K. D., Pappin, D. J., Aelst, L. V., & Tuveson, D. A. (2021). Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer. Proceedings of the National Academy of Sciences, 118(21). https://doi.org/10.1073/pnas.2016904118 Cite
Liquid chromatography with tandem mass spectrometry was used to analyze epoxyeicosatrienoic acids (EETs) in the lung tissues of patients with idiopathic pulmonary fibrosis and controls, and the function of 11,12-EET was evaluated in in vitro and in vivo in pulmonary fibrosis models.
[Environmental & Molecular Medicine]
7753456 ADPCTB34 items 1 apa default asc 1
Scientists developed a strategy that enabled single-cell lipidomic analysis with high structural specificity and classified four subtypes of human breast cancer cells.
6445212 QCHY7PGA items 1 apa default asc 1
Researchers analyzed the phytoconstituents in ethanolic Ajwa Dates Pulp Extract by liquid chromatography-mass spectrometry and investigated anticancer effects against MDA-MB-231 cells.
6445212 UXXMKNYH items 1 apa default asc 1
Using mass spectrometry, the authors characterized the myofilament chaperone-assisted selective autophagy interactome in the human heart and identified eight clients of BAG3-mediated protein turnover.
6445212 NR7PWVG8 items 1 apa default asc 1