The authors identified and isolated human iPSC-dopamine neurons with a TH-RFP reporter. Using liquid extraction surface analysis (LESA) coupled with high-resolution mass spectrometry they developed a high-throughput method for untargeted single cell lipid profiling.
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Snowden, S. G., Fernandes, H. J. R., Kent, J., Foskolou, S., Tate, P., Field, S. F., Metzakopian, E., & Koulman, A. (2020). Development and application of high-throughput single cell lipid profiling: a study of SNCA-A53T human dopamine neurons. IScience, 0(0). https://doi.org/10.1016/j.isci.2020.101703 Cite
Scientists combined data from secretome and proteome analysis using mass spectrometry with microarray data from mesenchymal transformed breast cancer cells to elucidate the drivers of epithelial-mesenchymal transition and cell invasion.
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Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling | Scientific Reports. (n.d.). Retrieved October 21, 2020, from https://www.nature.com/articles/s41598-020-74838-8 Cite
Wild-type EVI1 with S436 available for phosphorylation, but not non-phosphorylatable EVI1-S436A, conferred hematopoietic progenitor cell self-renewal and was associated with significantly higher organized transcriptional patterns.
[Cell Death & Disease]
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Paredes, R., Kelly, J. R., Geary, B., Almarzouq, B., Schneider, M., Pearson, S., Narayanan, P., Williamson, A., Lovell, S. C., Wiseman, D. H., Chadwick, J. A., Jones, N. J., Kustikova, O., Schambach, A., Garner, T., Amaral, F. M. R., Pierce, A., Stevens, A., Somervaille, T. C. P., … Meyer, S. (2020). EVI1 phosphorylation at S436 regulates interactions with CtBP1 and DNMT3A and promotes self-renewal. Cell Death & Disease, 11(10), 1–14. https://doi.org/10.1038/s41419-020-03099-0 Cite
The expression levels of CIB1 in lung adenocarcinoma tissues and adjacent normal tissues were examined by immunohistochemistry, and the relationship between CIB1 expression and patient clinicopathological characteristics was analyzed.
[Cell Death & Differentiation]
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Knockdown of CASZ1b via RNA interference increased the expression levels of the aldosterone-induced MR target genes epithelial Na+ channel-α and serum/glucocorticoid regulated kinase 1 by approximately twofold and 2.3-fold.
Researchers showed that cell-free DNA presence and clearance in plasma of healthy individuals played an indispensable role in immune system regulation.
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Mass spectrometry of endothelial cell culture media following P. aeruginosa infection allowed identification of multiple potential secreted modulators of beta amyloid, including cystatin C, gelsolin, and ApoJ/clusterin.
[Febs Open Bio]
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Balczon, R., Morrow, K. A., Leavesley, S., Francis, C. M., Stevens, T. C., Agwaramgbo, E., Williams, C., Stevens, R. P., Langham, G., Voth, S., Cioffi, E. A., Weintraub, S. E., & Stevens, T. (n.d.). Cystatin C regulates the cytotoxicity of infection-induced endothelial-derived β-amyloid. FEBS Open Bio, n/a(n/a). https://doi.org/10.1002/2211-5463.12997 Cite
Researchers performed a large-scale screen of electrophile and non-covalent fragments through a combined mass spectrometry and X-ray approach against the SARS-CoV-2 main protease, one of two cysteine viral proteases essential for viral replication.
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Douangamath, A., Fearon, D., Gehrtz, P., Krojer, T., Lukacik, P., Owen, C. D., Resnick, E., Strain-Damerell, C., Aimon, A., Ábrányi-Balogh, P., Brandão-Neto, J., Carbery, A., Davison, G., Dias, A., Downes, T. D., Dunnett, L., Fairhead, M., Firth, J. D., Jones, S. P., … Walsh, M. A. (2020). Crystallographic and electrophilic fragment screening of the SARS-CoV-2 main protease. Nature Communications, 11(1), 5047. https://doi.org/10.1038/s41467-020-18709-w Cite
In vitro human keratinocyte cell culture, ex vivo human skin organ explants and the eczema prone Nc/Tnd mouse were used as model systems to assess type-2 promoting immune responses to S. aureus.
[Journal of Allergy and Clinical Immunology]
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Kindi, A. A., Williams, H., Matsuda, K., Alkahtani, A. M., Saville, C., Bennett, H., Alshammari, Y., Tan, S. Y., O’Neill, C., Tanaka, A., Matsuda, H., Arkwright, P. D., & Pennock, J. L. (2020). Staphylococcus aureus Second Immunoglobulin-Binding Protein drives atopic dermatitis via IL-33. Journal of Allergy and Clinical Immunology, 0(0). https://doi.org/10.1016/j.jaci.2020.09.023 Cite
Scientists investigated the role of deubiquitinases (DUB) in regulating estrogen receptor α (ERα) in breast cancer. An RNAi loss-of-function screen in breast cancer cells targeting all DUB identified USP11 was a regulator of ERα transcriptional activity, which was further validated by assessment of direct transcriptional targets of ERα.
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Dwane, L., O’Connor, A. E., Das, S., Moran, B., Mulrane, L., Pinto-Fernandez, A., Ward, E., Blümel, A. M., Cavanagh, B. L., Mooney, B., Dirac, A. M., Jirström, K., Kessler, B. M., Chonghaile, T. N., Bernards, R., Gallagher, W. M., & O’Connor, D. P. (2020). A functional genomic screen identifies the deubiquitinase USP11 as a novel transcriptional regulator of ERα in breast cancer. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-20-0214 Cite
RNAi studies showed that reducing levels of METTL3, the active m6A methyltransferase, reduced global m6A levels and forced C2C12 myoblasts to prematurely differentiate.
[Cell Death Discovery]
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Gheller, B. J., Blum, J. E., Fong, E. H. H., Malysheva, O. V., Cosgrove, B. D., & Thalacker-Mercer, A. E. (2020). A defined N6-methyladenosine (m 6 A) profile conferred by METTL3 regulates muscle stem cell/myoblast state transitions. Cell Death Discovery, 6(1), 1–14. https://doi.org/10.1038/s41420-020-00328-5 Cite