organoids

[Nature Communications] Investigators established a midbrain organoid culture system to study the developmental trajectory from pluripotent stem cells to mature dopamine neurons.

[Communications Biology] The authors discuss promising Matrigel-free methods for the generation and maintenance of organoids that use decellularized ECM, synthetic hydrogels, or gel-forming recombinant proteins.

[Biomaterials] Human primary hepatocyte organoids and HepaRG spheroids showed more mature hepatocyte phenotype after adding fibronectin and fibrinogen to the culture system.

[Cell] In vivo, scientists identified a new intermediate pancreatic ductal adenocarcinoma transcriptional cell state and uncovered distinct site- and state-specific tumor microenvironments.

[PLoS One] Investigators attempted to better understand these structures by focusing on the choice of the best housekeeping gene to perform accurate molecular analysis on such a heterogeneous system.

[Journal of Medicinal Chemistry] AL-GDa62 preferentially induced apoptosis in CDH1–/– cells, and Cdh1–/– mammary and gastric organoids were significantly more sensitive to AL-GDa62 at low micromolar concentrations.