organoids

[Nature Cancer] Using a versatile platform for modulating neoantigen expression in colorectal cancer organoids and transplantation into the distal colon of mice, researchers showed that low expression precluded productive cross-priming and drove immediate T-cell dysfunction.

[Cell Stem Cell] The authors showed that SATB2, a colon-restricted chromatin factor, singularly preserved LGR5+ adult colonic stem cell and epithelial identity in mice and humans.

[Gastroenterology] Intestinal tissue from transgenic mice and patients were analyzed by histopathology and immunostaining. Human CRC cells and neoplastic murine organoids were genetically manipulated for functional studies.

[Medical Oncology] Using orthogonal flow cytometry analysis, scientists demonstrated that primary patient-derived cancer organoid maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor-related elements.

[Stem Cell Research & Therapy] The in vitro and in vivo cartilage and bone tissue formation potencies of human PSCs were investigated. The iPSCs were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids.

[Proceedings of the National Academy of Sciences of the United States of America] Scientists generated retinal organoids from achromatopsia patient iPSCs carrying ATF6 disease variants and from gene-edited ATF6 null human ESCs, and found that achromatopsia patient and ATF6 null retinal organoids failed to form cone structures concomitant with loss of cone phototransduction gene expression, while rod photoreceptors developed normally.