qRT-PCR

[Fundamental & Clinical Pharmacology] The changes in gene expression profiles of multidrug resistant prostate cancer cells that were established in response to docetaxel were determined using microarray analysis.

[Cell Cycle] A biotin-microRNA pull-down assay was performed to identify potential miRNAs. qRT-PCR was used to verify the KRT18 and miR-186-3p levels.

[Journal of Experimental & Clinical Cancer Research] Analysis of RNA sequencing and qRT-PCR revealed that miR-3189 increased and GLUT3 decreased by histone deacetylase 2 (HDAC2) knockdown.

[inflammopharmacology] Researchers investigated the effect of curcumin on vascular smooth muscle cells inflammation induced by lipopolysaccharide and its mechanism.

[Disease Markers] Ferroptosis may be involved in the occurrence and development of Ischemic heart disease (IHD). Primary cardiomyocytes were treated with H2O2 to simulate the IHD in vitro model.

[Aging] Scientists characterized a novel pseudogene-miRNA-mRNA network associated with PDAC progression using bioinformatics analysis. After screening by dreamBase and GEPIA, 12 up-regulated and 7 down-regulated differentially expressed pseudogenes were identified.