qRT-PCR

[Laboratory Investigation] Researchers revealed that hypoxia/reoxygenation (H/R) treated human cardiac myocytes showed a significantly decreased miR-185-5p expression and increased expression of CDK6 and ROR.

[Cell Death & Disease] HERC3 was indicated to inhibit the migration, invasion and metastasis in vitro and in vivo through transwell assays, wound healing assays and vivo experiments.

[Laboratory Investigation] Pulmonary artery smooth muscle cells were cultured in hypoxia condition for constructing a pulmonary arterial hypertension model in vitro. The expression of genes and proteins were determined by qRT-PCR and western bolt assays.

[Metabolic Brain Disease] CircRNA cyclin B1 (circCCNB1) knockdown reduced cyclin D1 (CCND1) expression in glioma cells and CCND1 overexpression bated the effect of circCCNB1 knockdown on glioma cell growth.

[Clinical and Translational Medicine] Rescue assays of cells overexpressing Metastasis-related in colon cancer 1 (MACC1)-AS1 and of cells silencing MACC1 were performed and cellular properties and proteins were assessed by the above-mentioned assays as well.

[Cancer Cell International] Molecular markers play an important role in predicting clinical outcomes in pancreatic adenocarcinoma patients. The 173 samples were divided into 3 categories and a 3-gene signature model was constructed.