single-cell RNA sequencing

[Nature] Scientists used organoid models of the human cerebral cortex to identify cell-type-specific developmental abnormalities that result from haploinsufficiency in three autism spectrum disorder risk genes—SUV420H1, ARID1B and CHD8—in multiple cell lines from different donors.

[Stem Cell Research & Therapy] Investigators discuss how single-cell RNA sequencing technologies contribute to tracing origin and lineage commitment of HSCs, profiling the bone marrow microenvironment and providing high-resolution dissection of malignant hematopoiesis, leading to exciting new findings in HSC biology.

[Nature Cancer] Scientists performed a systematic high-throughput combination drug screen and identified a synergistic interaction between the MEK inhibitor trametinib and the multi-kinase inhibitor nintedanib, which targeted KRAS-directed oncogenic signaling in mesenchymal PDAC.

[Scientific Reports] Researchers used global gene expression in human coronary artery endothelial cells to identify gene pathways and cellular processes in response to chemical hypoxia, oxidized lipids, IL-1β induced inflammation, oscillatory flow, and these combined stimuli.

[PLoS Biology] Researchers performed single-cell RNA-sequencing of colon-derived stromal cells and identified distinct classes of fibroblasts with gene signatures that were differentially regulated by chronic inflammation, including IL-11–producing inflammatory fibroblasts.

[Cell Reports] Researchers extended the transcriptomic analyses to incorporate single-cell assay for transposase-accessible chromatin sequencing, a powerful method used to characterize potential gene regulatory networks through the changes in accessible chromatin that accompany cell-state changes.