smooth muscle cells

[Circulation Journal] The expression of nuclear paraspeckle assembly transcript 1, miR-30d-5p and A disintegrin and metalloprotease 10 was measured by qRT-PCR and western blot. Functional experiments were conducted by using a CCK-8 assay, EDU assay, flow cytometry, western blot, ELISA, and commercial kits.

[Nature Communications] The authors developed a nitric oxide-eluting hydrogel coating for vascular stents inspired by the biological functions of nitric oxide for cardiovascular system.

[Biochemistry and Cell Biology] Wistar-Kyoto rats and spontaneously hypertensive rats were used to detect the expression of Angptl2. Angiotensin II stimulated vascular smooth muscle cells to mimic hypertension in vitro.

[Experimental and Molecular Medicine] Scientists identified and functionally validated a lncRNA using human atherosclerotic plaques. Human aortic samples were obtained from patients who underwent aortic surgery, and tissues were classified according to atherosclerotic plaques.

[Scientific Reports] To identify the heterogeneous responses of drug treatments, scientists created an in vitro experimental model system using vascular smooth muscle cells spheroids and developed a machine learning-based computational method called HETEROID.

[Experimental & Molecular Medicine] Researchers identified and functionally validated a long noncoding RNAs using human atherosclerotic plaques. Human aortic samples were obtained from patients who underwent aortic surgery, and tissues were classified according to atherosclerotic plaques.