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Prognostic Implications of the Immunohistochemical Expression of Perilipin 1 and Adipophilin in High-Grade Liposarcoma

[Journal Of Clinical Pathology] Scientists evaluated the association between perilipin 1 or adipophilin expression and prognosis in liposarcoma. 97 primary resection specimens were examined.

Pax6-Induced Proliferation and Differentiation of Bone Marrow Mesenchymal Stem Cells into Limbal Epithelial Stem Cells

[Stem Cells And Development] The authors developed a novel method to differentiate bone marrow mesenchymal stem cells into limbal epithelial stem cells-like cells using a culture medium and paired box 6 (Pax6) transfection.

Suspension Culture Improves iPSC Expansion and Pluripotency Phenotype

[Current Stem Cell Research & Therapy] Sendai virus transfection of human peripheral blood mononuclear cells was used to establish mycoplasma and virus free iPSC lines without common genetic duplications or deletions. iPSCs were then expanded under 2D planar and 3D suspension culture conditions.

iPSCs Ameliorate Hypoxia-Induced Autophagy and Atrophy in C2C12 Myotubes via the AMPK/ULK1 Pathway

[Biological Research] Investigators co-cultured iPSCs with C2C12 myoblasts using a Transwell nested system and placed them in a DG250 anaerobic workstation for oxygen deprivation for 24 hours.

Identification and Functional Evaluation of Alternative Splice Variants of Dax1 in Mouse Embryonic Stem Cells

[Stem Cells And Development] Researchers identified 18 new transcripts of Dax1 with putative protein-coding properties, and compared their proteins structures with known Dax1 protein.

On-Chip Real-Time Impedance Monitoring of hiPSC-Derived and Artificial Basement Membrane-Supported Endothelium

[Bioactive Materials] Researchers constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells (mTSCs) on implantation: microcarrier was prepared by sodium alginate using a droplet microfluidics system, and mTSCs were attached to the microcarrier surface with laminin modifications, called T(micro).

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