Publication

[Journal Of Dermatology] Epidermal high-mobility group box 1 (HMGB1) release from antitumor necrosis factor-alpha (TNF-α) treated, or doxycycline-inducible RIPK3 or Bak-expressing human keratinocyte cells was determined by western blot/ELISA.

[Cellular Signalling] Investigators explored the mechanism of action of a highly specific PI3K/mTOR inhibitor, and Mnk inhibitor, alone and in combination, as well as their effect on the viability and invasiveness of melanoma cells.

[Stem Cells And Development] Investigators established a replicative senescence model of MSCs by successive passages and compared the phenotypic changes between pre-senescent MSCs (Pre-Sen MSCs) and Sen MSCs, and found that Sen MSCs exhibited a diminished adipogenic and osteogenic differentiation potential.

[Metabolic Brain Disease] To investigate whether the conditioned medium of menstrual blood-derived endometrial stem cell could effectively relieve the symptoms of NPC1, researchers employed the CRISPR-Cas9 technique to successfully create a Npc1 gene knockout N2a cell line.

[Biotechniques] Researchers described novel device capable of delivering a direct current of electric field (EF)stimulation to cells cultured in vitro. This bioreactor was customized to simultaneously apply a direct-current EF to six individual cell culture wells, which reduced the amount of experimental time and minimized cost.

[Bone Research] The authors identified Cullin 4B as a critical regulator of MSC commitment. CUL4B is expressed in bone marrow MSCs (BMSCs) and downregulated with aging in mice and humans.