Publication

[Cell Proliferation] Scientists showed that apical-out organoids had drastically reduced proliferative potential, as evidenced by immunohistochemical staining and the incorporation of the thymidine analog EdU.

[Frontiers in Cell and Developmental Biology] For characterization of organoids’ response to single or multiple-cell ablation, researchers analyzed overall organoid survival and found that airway organoids were capable of efficiently repairing damage induced by femtosecond laser-based ablation of a single to ten cells within 24 h.

[Frontiers in Cell and Developmental Biology] The authors generated different homozygous deletions to knockout grainyhead-like 2 (Grhl2) in mouse embryonic stem cells, including some mimicking naturally occurring truncations in the dimerisation domain related to human deafness autosomal dominant type 28.

[Frontiers in Cardiovascular Medicine] Researchers reported the development of chamber-specific, vascularized hiPSC-derived cardiac microtissues, which allowed for the multi-parametric assessment of cardiac fibrosis.

[Frontiers in Oncology] Scientists generated organoids and an orthotopic mouse model from intestine tumors developed in a Msh2-deficient mouse model, and followed with a detailed characterization.

[Nucleic Acids Research] Investigators sought to identify novel targets to selectively kill BRCA1-mutant cancers. They reported that loss of RNF8 significantly protected Brca1-mutant mice against mammary tumorigenesis.