Tag Archives: western blot analysis

Podophyllotoxin Induces ROS-Mediated Apoptosis and Cell Cycle Arrest in Human Colorectal Cancer Cells via p38 MAPK Signaling

Researchers evaluated the anticancer effect of Podophyllotoxin on human colorectal cancer HCT116 cells and identified the detailed molecular mechanism.
[Biomolecules & Therapeutics]
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Involvement of Multiple Scavenger Receptors in Advanced Glycation End Product-Induced Vessel Tube Formation in Endothelial Cells

Researchers examined the involvement of advanced glycation end (AGE)-related receptors on AGE-induced angiogenesis in endothelial cells.
[Experimental Cell Research]
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Circ_0004018 Suppresses Cell Proliferation and Migration in Hepatocellular Carcinoma via miR-1197/PTEN/PI3K/AKT Signaling Pathway

Researchers conducted quantitative reverse transcription PCR to find that circ_0004018 was down-regulated in hepatocellular carcinoma cells. Western blot analysis was performed to detect the protein levels of phosphatase and tensin homologue and related factors of PI3K/AKT signaling pathway.
[Cell Cycle]
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Chlorothalonil Induces the Intestinal Epithelial Barrier Dysfunction in Caco-2 Cell-Based In Vitro Monolayer Model by Activating MAPK Pathway

Scientists used Caco-2 cell monolayers as an in vitro model of human intestinal epithelial barrier (IEB) to evaluate the toxicity of chlorothalonil exposure on the structure and function of IEB.
[Acta Biochimica et Biophysica Sinica]
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Rutin Inhibited the Advanced Glycation End Products-Stimulated Inflammatory Response and Extracellular Matrix Degeneration via Targeting TRAF-6 and BCL-2 Proteins in Mouse Model of Osteoarthritis

Scientists screened rutin (RT) for its activity against osteoarthritis with in vivo and in vitro models of osteoarthritis. Pretreatment of RT decreased the ECM degradation, inhibited expression of TRAF-6 and BCL-2 the NF-κB/MAPK pathway proteins.
[Aging]
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Histone Deacetylase HDAC4 Participates in the Pathological Process of Myocardial Ischemia-Reperfusion Injury via MEKK1/JNK Pathway by Binding to miR-206

Investigators defined the underlying role of histone deacetylase 4 (HDAC4) and miR-206 in the pathological process of myocardial ischemia-reperfusion injury (MIRI). Up-regulation of HDAC4 and down-regulation of miR-206 occurred in rat myocardial tissues and cardiomyocytes in MIRI.
[Cell Death Discovery]
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Hypoxia Increases KIAA1199/CEMIP Expression and Enhances Cell Migration in Pancreatic Cancer

Using quantitative real-time RT-PCR and western blot analysis, scientists analysed the changes in the expression of HA-synthesizing enzymesand hyaluronan HA-degrading enzymes in pancreatic ductal adenocarcinoma cell lines under hypoxic conditions.
[Scientific Reports]
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Bioglass Promotes Wound Healing by Inhibiting Endothelial Cell Pyroptosis through Regulation of the Connexin 43/Reactive Oxygen Species (ROS) Signaling Pathway

Researchers investigated the possible effect of bioactive glass (BG) on pyroptosis in wound healing both in vivo and in vitro and showed that BG could accelerate wound closure, granulation formation, collagen deposition, and angiogenesis.
[Laboratory Investigation]
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Pancreatic Cancer Cell-Derived MicroRNA-155-5p-Containing Extracellular Vesicles Promote Immune Evasion by Triggering EHF-Dependent Activation of Akt/NF-κB Signaling Pathway

Ectopic expression and knockdown experiments were conducted in pancreatic cancer (PC) cells, PC cells-derived EVs, or mouse xenograft model of PC.
[International Immunopharmacology]
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Serum-Derived Exosomes from House Dust Mite-Sensitized Guinea Pigs Contribute to Inflammation in BEAS-2B Cells via the TLR4-NF-κB Pathway

The authors investigated whether serum exosomes played a pro‑inflammatory role in bronchial epithelial cells and explored the underlying molecular mechanisms.
[Molecular Medicine Reports]
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Melatonin Restores Osteoporosis-Impaired Osteogenic Potential of Bone Marrow Mesenchymal Stem Cells and Alleviates Bone Loss through the HGF/PTEN/Wnt/β-Catenin Axis

Real-time qualitative PCR and Western blot analysis were conducted to determine expression of HGF, PTEN, and osteoblast differentiation-related genes in ovariectomy-induced osteoporosis mice and the isolated bone marrow MSCs.
[Therapeutic Advances in Chronic Disease]
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