Tag Archives: western blot analysis

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M2 Macrophages, but Not M1 Macrophages, Support Megakaryopoiesis by Upregulating PI3K-AKT Pathway Activity

Aberrant bone marrow-M1/M2 MФ polarization, characterized by increased M1 MФs and decreased M2 MФs and accompanied by impaired megakaryopoiesis-supporting abilities, was found in patients with thrombocytopenia post-allotransplant.

[Signal Transduction and Targeted Therapy]

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miR-141-5p Suppresses Vascular Smooth Muscle Cell Inflammation, Proliferation, and Migration via Inhibiting the HMGB1/NF-κB Pathway

Using human vascular smooth muscle cells (VSMCs) treated by oxidized low-density lipoprotein to establish an atherosclerosis cell model, researchers explored the functional roles and molecular mechanisms of miR-141-5p in VSMCs dysfunction.

[Journal of Biochemical and Molecular Toxicology]

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Isorhapontigenin Ameliorates Cerebral Ischemia/Reperfusion Injury via Modulating Kinase Cε/Nrf2/HO-1 Signaling Pathway

Focal cerebral ischemia-reperfusion injury model and primary cortical neurons were established an oxygen-glucose deprivation injury model. Neuronal cell viability was determined by MTT assay. Western blot analysis was determined for protein expression.

[Brain and Behavior]

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Exosomal Linc-ROR Mediates Crosstalk between Cancer Cells and Adipocytes to Promote Tumor Growth in Pancreatic Cancer

The effects of conditioned medium collected from dedifferentiated adipocytes on pancreatic cancer cell proliferation, motility, metastasis and epithelial-mesenchymal transition (EMT) were evaluated by western blot analysis, colony forming, real-time cell analysis, 5-ethynyl-2’-deoxyuridine, immunofluorescence, Transwell and wound healing assays in vitro.

[Molecular Therapy-Nucleic Acids]

Abstract

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Ndufs4 Ablation Decreases Synaptophysin Expression in Hippocampus

Researchers silenced Ndufs4 in Neuro-2a cells and we observed shorter neurite lengths with decreased expression of synaptophysin.

[Scientific Reports]

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Bone Marrow Mesenchymal Stem Cells Promote Prostate Cancer Cell Stemness via Cell–Cell Contact to Activate the Jagged1/Notch1 Pathway

Scientists investigated the effect of direct contact of prostate cancer (PCa) cells with mesenchymal stem cells on the stemness of PCa and its mechanisms.

[Cell and Bioscience]

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Spermidine Inhibits Vascular Calcification in Chronic Kidney Disease through Modulation of SIRT1 Signaling Pathway

Alizarin red staining and quantification of calcium content showed that spermidine treatment markedly reduced mineral deposition in both rat and human vascular smooth muscle cells (VSMCs) under osteogenic conditions.

[Aging Cell]

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LncRNA SLCO4A1-AS1 Modulates Colon Cancer Stem Cell Properties by Binding to miR-150-3p and Positively Regulating SLCO4A1

The authors explored the specific role of solute carrier organic anion transporter family member 4A1-AS1 (SLCO4A1-AS1) in colon CSCs.

[Laboratory Investigation]

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Apoptosis-Mediated Anticancer Activity in Prostate Cancer Cells of a Chestnut Honey (Castanea sativa L.) Quinoline–Pyrrolidine Gamma-Lactam Alkaloid

Scientists studied the in vitro inhibitory properties of the tryptophan-derived endogenous metabolite kynurenic acid and of the lactam form of 3–2′-pyrrilonidinyl-kynurenic acid, alkaloids usually present in combination in chestnut honey in two castration-resistant prostate cancer cell lines.

[Amino Acids]

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Patient-Specific induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells as a Model to Study Autosomal Recessive Hypercholesterolemia

Investigators aimed to validate patient-specific iPSC-derived hepatocyte-like cells as an appropriate tool to model autosomal recessive hypercholesterolemia disease.

[Stem Cells and Development]

Abstract