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western blot analysis

Exosomal Linc-ROR Mediates Crosstalk between Cancer Cells and Adipocytes to Promote Tumor Growth in Pancreatic Cancer

[Molecular Therapy-Nucleic Acids] The effects of conditioned medium collected from dedifferentiated adipocytes on pancreatic cancer cell proliferation, motility, metastasis and epithelial-mesenchymal transition (EMT) were evaluated by western blot analysis, colony forming, real-time cell analysis, 5-ethynyl-2’-deoxyuridine, immunofluorescence, Transwell and wound healing assays in vitro.

Ndufs4 Ablation Decreases Synaptophysin Expression in Hippocampus

[Scientific Reports] Researchers silenced Ndufs4 in Neuro-2a cells and we observed shorter neurite lengths with decreased expression of synaptophysin.

Bone Marrow Mesenchymal Stem Cells Promote Prostate Cancer Cell Stemness via Cell–Cell Contact to Activate the Jagged1/Notch1 Pathway

[Cell and Bioscience] Scientists investigated the effect of direct contact of prostate cancer (PCa) cells with mesenchymal stem cells on the stemness of PCa and its mechanisms.

Spermidine Inhibits Vascular Calcification in Chronic Kidney Disease through Modulation of SIRT1 Signaling Pathway

[Aging Cell] Alizarin red staining and quantification of calcium content showed that spermidine treatment markedly reduced mineral deposition in both rat and human vascular smooth muscle cells (VSMCs) under osteogenic conditions.

LncRNA SLCO4A1-AS1 Modulates Colon Cancer Stem Cell Properties by Binding to miR-150-3p and Positively Regulating SLCO4A1

[Laboratory Investigation] The authors explored the specific role of solute carrier organic anion transporter family member 4A1-AS1 (SLCO4A1-AS1) in colon CSCs.

Apoptosis-Mediated Anticancer Activity in Prostate Cancer Cells of a Chestnut Honey (Castanea sativa L.) Quinoline–Pyrrolidine Gamma-Lactam Alkaloid

[Amino Acids] Scientists studied the in vitro inhibitory properties of the tryptophan-derived endogenous metabolite kynurenic acid and of the lactam form of 3–2′-pyrrilonidinyl-kynurenic acid, alkaloids usually present in combination in chestnut honey in two castration-resistant prostate cancer cell lines.

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