Keep Current with the Latest in Cell Biology Research

Spatially-Resolved Single-Cell Assessment of Pancreatic Cancer Expression Subtypes Reveals Co-Expressor Phenotypes and Extensive Intra-Tumoral Heterogeneity

[Cancer Research] To provide a deeper understanding of PDAC subtypes, scientists developed a multiplex immunofluorescence pipeline that quantifies protein expression of six PDAC subtype markers and permited spatially resolved, single-cell interrogation of pancreatic tumors from resection specimens and core needle biopsies.

Creating a Kidney Organoid-Vasculature Interaction Model Using a Novel Organ-on-Chip System

[Scientific Reports] Scientists reported a system supported culturing of kidney organoids, which presented nephron structures. They showed that organoids cultured on chip showed increased maturation of endothelial populations based on a colocalization analysis of endothelial markers.

KIN17 Promotes Cell Migration and Invasion through Stimulating the TGF-β/Smad2 Pathway in Hepatocellular Carcinoma

[Molecular Carcinogenesis] The transwell, wound-healing assay was employed to determine the effects of KIN17 on migration and invasion of HCC cells in vitro.

In Vitro Production of Infectious Plasmodium falciparum Sporozoites

[Nature] Plasmodium falciparum (Pf) sporozoites invaded human hepatocytes in culture and developed to mature liver-stage schizonts expressing Pf merozoite surface protein in numbers comparable to those manufactured in mosquitoes.

MKL-1-Induced PINK1-AS Overexpression Contributes to the Malignant Progression of Hepatocellular Carcinoma via ALDOA-Mediated Glycolysis

[Scientific Reports] The authors investigated the expression pattern of ALDOA in hepatocellular carcinoma and its biological role in tumour progression. Bioinformatics analysis, western blot and RT-qPCR were performed to detect the relative expression of ALDOA in HCC tissues and cell lines.

LncRNA CCAT2, Involving miR-34a/TGF-β1/Smad4 Signaling, Regulate Hepatic Stellate Cells Proliferation

[Scientific Reports] The interaction between CCAT2 and miR-34a-5p was checked by dual luciferase assay. The effects of CCAT2 and miR-34a-5p on cell proliferation and apoptosis were verified by MTT assay, colony formation assay, and flow cytometry assay.

“Organ-in-a-Column” Coupled On-line with Liquid Chromatography-Mass Spectrometry

[Analytical Chemistry] Researchers loaded liquid chromatography column housings with induced pluripotent stem cell derived liver organoids and coupled the “organ-in-a-column” units on-line with liquid chromatography-mass spectrometry.

Exosomal microRNAs in the DLK1-DIO3 Imprinted Region Derived from Cancer-Associated Fibroblasts Promote Progression of Hepatocellular Carcinoma by Targeting Hedgehog Interacting Protein

[BMC Gastroenterology] Exosomes were isolated by ultracentrifugation, and characterized by transmission electron microscopy, nanoflow cytometry, and western blot assay. The internalization of exosomes by HCC cells was observed under a fluorescence microscope.

Reduced Klotho Expression and Its Prognostic Significance in Canine Hepatocellular Carcinoma

[Veterinary and Comparative Oncology] The authors aimed to confirm Klotho expression in normal canine liver tissues using western blotting and immunohistochemistry, and whether the expression differed in non-neoplastic liver disease and HCC.

Increased CHCHD2 Expression Promotes Liver Fibrosis in Nonalcoholic Steatohepatitis via Notch/Osteopontin Signaling

[JCI Insight] Coiled-coil-helix-coiled-coil-helix domain-containing (CHCHD2)-overexpressing hepatocytes activated hepatic stellate cells by upregulating osteopontin levels, a downstream mediator of Notch signals.

Exosomal miR-628-5p from M1 Polarized Macrophages Hinders m6A Modification of circFUT8 to Suppress Hepatocellular Carcinoma Progression

[Cellular & Molecular Biology Letters] Researchers investigated the function of circFUT8 in HCC and its interaction with exosomes, M1 macrophages, and m6A.

Metformin in Therapeutic Applications in Human Diseases: Its Mechanism of Action and Clinical Study

[Molecular Biomedicine] Scientists summarize research developments on the mechanism of action and clinical evidence of metformin and discusses its therapeutic potential and clinical safety.

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