Keep Current with the Latest in Cell Biology Research
[Nature Communications] In this phase III clinical trial, 148 patients with resected stage IIIB/C melanoma were randomized to adjuvant treatment with natural dendritic cells (nDCs) or placebo. Active treatment consisted of intranodally injected autologous CD1c+ conventional and plasmacytoid DCs loaded with tumor antigens.
[Journal Of Experimental Medicine] Investigators performed an unbiased network analysis of mouse skin during invasive S. aureus infection and revealed a dominant role for CXCL12+ fibroblast subsets in neutrophil communication.
[Cancer Cell] Scientists enhanced CAR-T cell potency by knocking out CD39, CD73, or adenosine receptor 2a but observed only modest effects. In contrast, overexpression of Ado deaminase, which metabolized Ado to inosine (INO), induced stemness and enhanced CAR-T functionality.
A Versatile CRISPR-Cas13d Platform for Multiplexed Transcriptomic Regulation and Metabolic Engineering in Primary Human T Cells
[Cell] To address these challenges, the authors developed multiplexed effector guide arrays (MEGA), a platform for programmable and scalable regulation of the T cell transcriptome using the RNA-guided, RNA-targeting activity of CRISPR-Cas13d. MEGA enabled quantitative, reversible, and massively multiplexed gene knockdown in primary human T cells without targeting or cutting genomic DNA.
PRL-Mediated STAT5B/ARRB2 Pathway Promotes the Progression of Prostate Cancer through the Activation of MAPK Signaling
[Cell Death & Disease] The authors found heterogeneous expression of the prolactin (PRLR) in clinical prostate samples. The VCaP and 22Rv1 cells exhibited PRLR expression. Among the downstream proteins, STAT5B was the dominant subtype in clinical samples and cell lines.
A Novel HDAC6 Inhibitor Interferes Microtubule Dynamics and Spindle Assembly Checkpoint and Sensitizes Cisplatin-Induced Apoptosis in Castration-Resistant Prostate Cancer
[Prostate] Cell proliferation was examined in CRPC PC-3 and DU-145 cells using sulforhodamine B assay and anchorage-dependent colony formation assay. Flow cytometric analysis of propidium iodide staining was used to determine cell-cycle progression.
[International Journal Of Biochemistry & Cell Biology] The human prostate stromal cell line (WPMY-1), rat prostate epithelial cells, human prostate tissues and two BPH rat models were employed in this study.
[Trends In Cancer] De novo post-translational acetylation of the AR, HOXB13, and H2A at K609, K13, and K130, respectively, and phosphorylation of H4 at Y88 have emerged as key epigenetic modifications associated with castration-resistant prostate cancer.
Novel Estrogen Receptor β/Histone Deacetylase Dual-Targeted Near-Infrared Fluorescent Probes as Theranostic Agents for Imaging and Treatment of Prostate Cancer
[European Journal Of Medicinal Chemistry] Scientists investigated the design and synthesis of the first estrogen receptor (ERβ) and histone deacetylases dual-target near-infrared fluorescent probe with both imaging capacity and antitumor activity for prostate cancer.
Celcuity Announces First Patient Dosed in Phase Ib/2 CELC-G-201 Clinical Trial of Gedatolisib for the Treatment of Metastatic Castration Resistant Prostate Cancer
[Celcuity, Inc. (GlobeNewswire, Inc.)] Celcuity, Inc. announced that the first patient has been dosed in its Phase Ib/II study evaluating gedatolisib in combination with Nubeqa®, an approved androgen receptor inhibitor, for the treatment of patients with metastatic castration resistant prostate cancer.
[International Journal Of Urology] Scientists focus on multifaceted resistance mechanisms, including androgen receptor overexpression, splice variants, missense mutations, the involvement of the glucocorticoid receptor, and alterations in coregulators and transcription factors, revealing their roles in castration-resistant prostate cancer progression.
Development of Certain Benzylidene Coumarin Derivatives as Anti-Prostate Cancer Agents Targeting EGFR and PI3Kβ Kinases
[Journal Of Enzyme Inhibition And Medicinal Chemistry] Novel coumarin derivatives were synthesised and tested for their cytotoxicity against human cancer cells. Compounds 5, 4b, and 4a possessed potent cytotoxic activity against PC-3 cells with IC50 3.56, 8.99, and 10.22 µM, respectively.